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- Direct interaction between the transcription factors CadC and OmpR involved in the acid stress response of Salmonella enterica
- Yong Heon Lee , Ji Hye Kim
- J. Microbiol. 2017;55(12):966-972. Published online December 7, 2017
- DOI: https://doi.org/10.1007/s12275-017-7410-7
- 54 View
- 0 Download
- 7 Crossref
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Abstract
-
In Salmonella enterica serovar Typhimurium, the acid-sensing
regulator CadC activates transcription of the cadBA operon
which contributes to the acid tolerance response. The
DNA-binding response regulator OmpR in two-component
regulatory system with EnvZ binds to its own promoter for
autoinduction. We previously reported that CadC exerts a
negative influence on ompR transcription during acid adaptation.
However, its underlying mechanisms remain to be elucidated.
Here we show that the level of OmpR protein is gradually
reduced by a gradual increase in the CadC level using
an arabinose-inducible expression system, indicating there
exists a negative correlation between the expression levels of
two transcription factors. To explore the molecular basis for
OmpR repression by CadC, we performed in vitro binding assays
and determined that CadC directly interacts with OmpR.
We further show that inactivation of cadC inhibits transcription
of the fliC gene, which encodes the major flagellar subunit,
result
ing in impaired flagellar motility under acid-adaptation conditions. Together, our findings suggest that CadC may repress autoinduction of the OmpR response regulator through their direct interaction. -
Citations
Citations to this article as recorded by
- Isolation and characterization of resistant variants of Salmonella Typhimurium after sequential exposure to plasma activated water (PAW)
Elisa Pagán, Foteini Pavli, Sarah Happiette, Daniel Berdejo, Ruben Gatt, Rafael Pagán, Vasilis Valdramidis, Diego García-Gonzalo
Innovative Food Science & Emerging Technologies.2024; 93: 103633. CrossRef -
A current insight into
Salmonella’s
inducible acid resistance
Alkmini Gavriil, Ilias Giannenas, Panagiotis N. Skandamis
Critical Reviews in Food Science and Nutrition.2024; : 1. CrossRef - Molecular mechanism of proteolytic cleavage-dependent activation of CadC-mediated response to acid in E. coli
Min Chen, Ye Shang, Wenhao Cui, Xiaomeng Wang, Jiakun Zhu, Hongjie Dong, Hongwei Wang, Tiantian Su, Weiwei Wang, Kundi Zhang, Bingqing Li, Sujuan Xu, Wei Hu, Fengyu Zhang, Lichuan Gu
Communications Biology.2024;[Epub] CrossRef - Transmembrane Transcription Regulators Are Widespread in Bacteria and Archaea
Lucas M. Demey, Vadim M. Gumerov, Jiawei Xing, Igor B. Zhulin, Victor J. DiRita, Beile Gao
Microbiology Spectrum.2023;[Epub] CrossRef -
Host acid signal controls
Salmonella
flagella biogenesis through CadC-YdiV axis
Weiwei Wang, Yingying Yue, Min Zhang, Nannan Song, Haihong Jia, Yuanji Dai, Fengyu Zhang, Cuiling Li, Bingqing Li
Gut Microbes.2022;[Epub] CrossRef - Recent advances in understanding the effect of acid-adaptation on the cross-protection to food-related stress of common foodborne pathogens
Ricardo A. Wu, Hyun-Gyun Yuk, Donghong Liu, Tian Ding
Critical Reviews in Food Science and Nutrition.2022; 62(26): 7336. CrossRef - Metabolic adaptability shifts of cell membrane fatty acids of Komagataeibacter hansenii HDM1-3 improve acid stress resistance and survival in acidic environments
Yuanjing Li, Pengfei Yan, Qingyun Lei, Bingyu Li, Yue Sun, Shuangfei Li, Hong Lei, Ning Xie
Journal of Industrial Microbiology and Biotechnology.2019; 46(11): 1491. CrossRef
- Isolation and characterization of resistant variants of Salmonella Typhimurium after sequential exposure to plasma activated water (PAW)
Research Support, Non-U.S. Gov't
- The Changes of Proteomes Components of Helicobacter pylori in Response to Acid Stress without Urea
- Chunhong Shao , Qunye Zhang , Wei Tang , Wei Qu , Yabin Zhou , Yundong Sun , Han Yu , Jihui Jia
- J. Microbiol. 2008;46(3):331-337. Published online July 5, 2008
- DOI: https://doi.org/10.1007/s12275-008-0062-x
- 40 View
- 0 Download
- 15 Scopus
-
Abstract
- Acid stress is the most obvious challenge Helicobacter pylori encounters in human stomach. The urease system is the basic process used to maintain periplasmic and cytoplasmic pH near neutrality when H. pylori is exposed to acidic condition. However, since the urea concentration in gastric juice is approximately 1 mM, considered possibly insufficient to ensure the survival of H. pylori, it is postulated that additional mechanisms of pH homeostasis may contribute to the acid adaptation in H. pylori. In order to identify the acid-related proteins other than the urease system we have compared the proteome profiles of H. pylori strain 26695 exposed to different levels of external pH (7.4, 6.0, 5.0, 4.0, 3.0, and 2.0) for 30 min in the absence of urea using 2-DE. Differentially expressed proteins were identified by MALDI-TOF-TOF-MS analysis, which turned out to be 36 different proteins. The functions of these proteins included ammonia production, molecular chaperones, energy metabolism, cell envelope, response regulator and some proteins with unknown function. SOM analysis indicated that H. pylori responds to acid stress through multi-mechanisms involving many proteins, which depend on the levels of acidity the cells encounter.
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