Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 46(3); 2008 > Article
Research Support, Non-U.S. Gov't
The Changes of Proteomes Components of Helicobacter pylori in Response to Acid Stress without Urea
Chunhong Shao 1, Qunye Zhang 2, Wei Tang 1, Wei Qu 1, Yabin Zhou 1, Yundong Sun 1, Han Yu 1, Jihui Jia 1,2
Journal of Microbiology 2008;46(3):331-337.
DOI: https://doi.org/10.1007/s12275-008-0062-x
Published online: July 5, 2008
1Department of Microbiology, School of Medicine, Shandong University, Jinan 250012, P. R. China, 2Key Lab for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan 250012, P. R. China
Corresponding author:  Jihui Jia , Tel: 86-531-8838-2672, 
Received: 4 March 2008   • Accepted: 13 April 2008
prev next
  • 116 Views
  • 0 Download
  • 13 Crossref
  • 15 Scopus
Full Article

Abstract

Acid stress is the most obvious challenge Helicobacter pylori encounters in human stomach. The urease system is the basic process used to maintain periplasmic and cytoplasmic pH near neutrality when H. pylori is exposed to acidic condition. However, since the urea concentration in gastric juice is approximately 1 mM, considered possibly insufficient to ensure the survival of H. pylori, it is postulated that additional mechanisms of pH homeostasis may contribute to the acid adaptation in H. pylori. In order to identify the acid-related proteins other than the urease system we have compared the proteome profiles of H. pylori strain 26695 exposed to different levels of external pH (7.4, 6.0, 5.0, 4.0, 3.0, and 2.0) for 30 min in the absence of urea using 2-DE. Differentially expressed proteins were identified by MALDI-TOF-TOF-MS analysis, which turned out to be 36 different proteins. The functions of these proteins included ammonia production, molecular chaperones, energy metabolism, cell envelope, response regulator and some proteins with unknown function. SOM analysis indicated that H. pylori responds to acid stress through multi-mechanisms involving many proteins, which depend on the levels of acidity the cells encounter.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    The Changes of Proteomes Components of Helicobacter pylori in Response to Acid Stress without Urea
    J. Microbiol. 2008;46(3):331-337.   Published online July 5, 2008
    DOI: https://doi.org/10.1007/s12275-008-0062-x
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP