The Changes of Proteomes Components of Helicobacter pylori in Response to Acid Stress without Urea

Chunhong Shao; Qunye Zhang; Wei Tang; Wei Qu; Yabin Zhou; Yundong Sun; Han Yu; Jihui Jia

doi:10.1007/s12275-008-0062-x

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Research Support, Non-U.S. Gov't
The Changes of Proteomes Components of Helicobacter pylori in Response to Acid Stress without Urea: Chunhong Shao ¹, Qunye Zhang ², Wei Tang ¹, Wei Qu ¹, Yabin Zhou ¹, Yundong Sun ¹, Han Yu ¹, Jihui Jia ^1,2; Journal of Microbiology 2008;46(3):331-337.
DOI: https://doi.org/10.1007/s12275-008-0062-x
Published online: July 5, 2008

¹Department of Microbiology, School of Medicine, Shandong University, Jinan 250012, P. R. China, ²Key Lab for Experimental Teratology of Chinese Ministry of Education, School of Medicine, Shandong University, Jinan 250012, P. R. China

Corresponding author: Jihui Jia , Tel: 86-531-8838-2672,

Received: 4 March 2008 • Accepted: 13 April 2008

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Abstract

Acid stress is the most obvious challenge Helicobacter pylori encounters in human stomach. The urease system is the basic process used to maintain periplasmic and cytoplasmic pH near neutrality when H. pylori is exposed to acidic condition. However, since the urea concentration in gastric juice is approximately 1 mM, considered possibly insufficient to ensure the survival of H. pylori, it is postulated that additional mechanisms of pH homeostasis may contribute to the acid adaptation in H. pylori. In order to identify the acid-related proteins other than the urease system we have compared the proteome profiles of H. pylori strain 26695 exposed to different levels of external pH (7.4, 6.0, 5.0, 4.0, 3.0, and 2.0) for 30 min in the absence of urea using 2-DE. Differentially expressed proteins were identified by MALDI-TOF-TOF-MS analysis, which turned out to be 36 different proteins. The functions of these proteins included ammonia production, molecular chaperones, energy metabolism, cell envelope, response regulator and some proteins with unknown function. SOM analysis indicated that H. pylori responds to acid stress through multi-mechanisms involving many proteins, which depend on the levels of acidity the cells encounter.

Keywords: Helicobacter pylori;acid stress;comparative proteomics

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The Changes of Proteomes Components of Helicobacter pylori in Response to Acid Stress without Urea

J. Microbiol. 2008;46(3):331-337. Published online July 5, 2008

DOI: https://doi.org/10.1007/s12275-008-0062-x

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