MSK
Search
- Page Path
- HOME > Search
Journal Article
- The assessment of host and bacterial proteins in sputum from active pulmonary tuberculosis
- Hsin-Chih Lai , Yu-Tze Horng , Pen-Fang Yeh , Jann-Yuan Wang , Chin-Chung Shu , Jang-Jih Lu , Jen-Jyh Lee , Po-Chi Soo
- J. Microbiol. 2016;54(11):761-767. Published online October 29, 2016
- DOI: https://doi.org/10.1007/s12275-016-6201-x
- 47 View
- 0 Download
- 2 Crossref
-
Abstract
- Pulmonary tuberculosis (TB) is caused by Mycobacterium tuberculosis. The protein composition of sputum may reflect the immune status of the lung. This study aimed to evaluate the protein profiles in spontaneous sputum samples from patients with active pulmonary TB. Sputum samples were collected from patients with pulmonary TB and healthy controls. Western blotting was used to analyze the amount of interleukin 10 (IL-10), interferon-gamma (IFN-γ), IL-25, IL- 17, perforin-1, urease, albumin, transferrin, lactoferrin, adenosine deaminase (also known as adenosine aminohydrolase, or ADA), ADA-2, granzyme B, granulysin, and caspase- 1 in sputum. Results of detection of IL-10, IFN-γ, perforin- 1, urease, ADA2, and caspase-1, showed relatively high specificity in distinguishing patients with TB from healthy controls, although sensitivities varied from 13.3% to 66.1%. By defining a positive result as the detection of any two proteins in sputum samples, combined use of transferrin and urease as markers increased sensitivity to 73.2% and specificity to 71.1%. Furthermore, we observed that the concentration of transferrin was proportional to the number of acidfast bacilli detected in sputum specimens. Detection of sputum transferrin and urease was highly associated with pulmonary TB infection. In addition, a high concentration of transferrin detected in sputum might correlate with active TB infection. This data on sputum proteins in patients with TB may aid in the development of biomarkers to assess the severity of pulmonary TB.
-
Citations
Citations to this article as recorded by
- From simple to complex: Protein‐based biomarker discovery in tuberculosis
Zaynab Mousavian, Gunilla Källenius, Christopher Sundling
European Journal of Immunology.2023;[Epub] CrossRef - Interleukin 8 and Pentaxin (C-Reactive Protein) as Potential New Biomarkers of Bovine Tuberculosis
Xintao Gao, Xiaoyu Guo, Ming Li, Hong Jia, Weidong Lin, Lichun Fang, Yitong Jiang, Hongfei Zhu, Zhifang Zhang, Jiabo Ding, Ting Xin, Brad Fenwick
Journal of Clinical Microbiology.2019;[Epub] CrossRef
- From simple to complex: Protein‐based biomarker discovery in tuberculosis
First
Prev
TOP
