Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
1 "alkalitolerant"
Filter
Filter
Article category
Keywords
Publication year
Review
Recent Advances in CRISPR‑Cas Technologies for Synthetic Biology
Song Hee Jeong , Ho Joung Lee , Sang Jun Lee
J. Microbiol. 2023;61(1):13-36.   Published online February 1, 2023
DOI: https://doi.org/10.1007/s12275-022-00005-5
  • 12 View
  • 0 Download
  • 5 Citations
AbstractAbstract
With developments in synthetic biology, “engineering biology” has emerged through standardization and platformization based on hierarchical, orthogonal, and modularized biological systems. Genome engineering is necessary to manufacture and design synthetic cells with desired functions by using bioparts obtained from sequence databases. Among various tools, the CRISPR-Cas system is modularly composed of guide RNA and Cas nuclease; therefore, it is convenient for editing the genome freely. Recently, various strategies have been developed to accurately edit the genome at a single nucleotide level. Furthermore, CRISPR-Cas technology has been extended to molecular diagnostics for nucleic acids and detection of pathogens, including disease-causing viruses. Moreover, CRISPR technology, which can precisely control the expression of specific genes in cells, is evolving to find the target of metabolic biotechnology. In this review, we summarize the status of various CRISPR technologies that can be applied to synthetic biology and discuss the development of synthetic biology combined with CRISPR technology in microbiology.

Journal of Microbiology : Journal of Microbiology
TOP