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- [MINIREVIEW] Progress of analytical tools and techniques for human gut microbiome research
- Eun-Ji Song , Eun-Sook Lee , Young-Do Nam
- J. Microbiol. 2018;56(10):693-705. Published online September 28, 2018
- DOI: https://doi.org/10.1007/s12275-018-8238-5
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Abstract
- Massive DNA sequencing studies have expanded our insights
and understanding of the ecological and functional
characteristics of the gut microbiome. Advanced sequencing
technologies allow us to understand the close association of
the gut microbiome with human health and critical illnesses.
In the future, analyses of the gut microbiome will provide
key information associating with human individual health,
which will help provide personalized health care for diseases.
Numerous molecular biological analysis tools have been rapidly
developed and employed for the gut microbiome researches;
however, methodological differences among researchers
lead to inconsistent data, limiting extensive share of
data. It is therefore very essential to standardize the current
method
ologies and establish appropriate pipelines for human gut microbiome research. Herein, we review the methods and procedures currently available for studying the human gut microbiome, including fecal sample collection, metagenomic DNA extraction, massive DNA sequencing, and data analyses with bioinformatics. We believe that this review will contribute to the progress of gut microbiome research in the clinical and practical aspects of human health.
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