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- Monitoring Nutrient Impact on Bacterial Community Composition during Bioremediation of Anoxic PAH-Contaminated Sediment
- Myungsu Kim , Seung Seob Bae , Mijin Seol , Jung-Hyun Lee , Young-Sook Oh
- J. Microbiol. 2008;46(6):615-623. Published online December 24, 2008
- DOI: https://doi.org/10.1007/s12275-008-0097-z
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Abstract
- Marine harbor sediments are frequently polluted with significant amount of polycyclic aromatic hydrocarbons (PAHs) some of which are naturally toxic, recalcitrant, mutagenic, and carcinogenic. To stimulate biodegradation of PAHs in PAH-contaminated sediments collected from near Gwangyang Bay, Korea, lactate was chosen as a supplementary carbonaceous substrate. Sediment packed into 600 ml air-tight jar was either under no treatment condition or lactate amended condition (1%, w/v). Microbial community composition was monitored by bacteria-specific and archaea-specific PCR-terminal restriction fragment length polymorphism (T-RFLP), in addition to measuring the residual PAH concentration. Results showed that lactate amendment enhanced biodegradation rate of PAHs in the sediment by 4 to 8 times, and caused a significant shift in archaebacterial community in terms of structure and diversity with time. Phylogenetic analysis of 23 archaeal clones with distinctive RFLP patterns among 288 archaeal clones indicated that majority of the archaeal members were closest to unculturable environmental rDNA clones from hydrocarbon-contaminated and/or methanogenesis-bearing sediments. Lactate amendment led to the enrichment of some clones that were most closely related to PAH-degrading Methanosarcina species. These results suggest a possible contribution of methanogenic community to PAH degradation and give us more insights on how to effectively remediate PAH-contaminated sediments.
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Citations
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- Effects of electron acceptors and donors on anaerobic biodegradation of PAHs in marine sediments
Qingguo Chen, Zhenzhen Li, Yu Chen, Mei Liu, Qiao Yang, Baikang Zhu, Jun Mu, Lijuan Feng, Zhi Chen
Marine Pollution Bulletin.2024; 199: 115925. CrossRef - Electrical current generation from a continuous flow macrophyte biocathode sediment microbial fuel cell (mSMFC) during the degradation of pollutants in urban river sediment
Felix Tetteh Kabutey, Jing Ding, Qingliang Zhao, Philip Antwi, Frank Koblah Quashie
Environmental Science and Pollution Research.2020; 27(28): 35364. CrossRef - Application of in situ Solid-Phase Microextraction on Mediterranean Sponges for Untargeted Exometabolome Screening and Environmental Monitoring
Barbara Bojko, Bora Onat, Ezel Boyaci, Eleftheria Psillakis, Thanos Dailianis, Janusz Pawliszyn
Frontiers in Marine Science.2019;[Epub] CrossRef - Effect of carbonyl cyanide m-chlorophenylhydrazone (CCCP) on microbial activity and polycyclic aromatic hydrocarbons (PAH) degradation in contaminated river sediments
G. Patricia Johnston, Z. Kalik, C. G. Johnston
Environmental Earth Sciences.2016;[Epub] CrossRef - Effects of polycyclic aromatic hydrocarbons on microbial community structure and PAH ring hydroxylating dioxygenase gene abundance in soil
Przemyslaw Sawulski, Nicholas Clipson, Evelyn Doyle
Biodegradation.2014; 25(6): 835. CrossRef - Anthracene biodegradation under nitrate-reducing condition and associated microbial community changes
Ying Wang, Rui Wan, Shuying Zhang, Shuguang Xie
Biotechnology and Bioprocess Engineering.2012; 17(2): 371. CrossRef - Molecular characterization of phenanthrene-degrading methanogenic communities in leachate-contaminated aquifer sediment
S. Y. Zhang, Q. F. Wang, S. G. Xie
International Journal of Environmental Science and Technology.2012; 9(4): 705. CrossRef - Increment in Anaerobic Hydrocarbon Degradation Activity of Halic Bay Sediments via Nutrient Amendment
Mustafa Kolukirik, Orhan Ince, Bahar K. Ince
Microbial Ecology.2011; 61(4): 871. CrossRef - Revealing archaeal diversity patterns and methane fluxes in Admiralty Bay, King George Island, and their association to Brazilian Antarctic Station activities
C.R. Nakayama, E. Kuhn, A.C.V. Araújo, P.C. Alvalá, W.J. Ferreira, R.F. Vazoller, V.H. Pellizari
Deep Sea Research Part II: Topical Studies in Oceanography.2011; 58(1-2): 128. CrossRef - Bioremediation of marine sediments contaminated by hydrocarbons: Experimental analysis and kinetic modeling
Francesca Beolchini, Laura Rocchetti, Francesco Regoli, Antonio Dell’Anno
Journal of Hazardous Materials.2010; 182(1-3): 403. CrossRef
- Effects of electron acceptors and donors on anaerobic biodegradation of PAHs in marine sediments
Research Support, Non-U.S. Gov't
- Enhancing the Intrinsic Bioremediation of PAH-Contaminated Anoxic Estuarine Sediments with Biostimulating Agents
- Quang-Dung Bach , Sang-Jin Kim , Sung-Chan Choi , Young-Sook Oh
- J. Microbiol. 2005;43(4):319-324.
- DOI: https://doi.org/2259 [pii]
- 39 View
- 0 Download
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Abstract
- Estuarine sediments are frequently polluted with hydrocarbons from fuel spills and industrial wastes. Polycyclic aromatic hydrocarbons (PAHs) are components of these contaminants that tend to accumulate in the sediment due to their low aqueous solubility, low volatility, and high affinity for particulate matter. The toxic, recalcitrant, mutagenic, and carcinogenic nature of these compounds may require aggressive treatment to remediate polluted sites effectively. In petroleum-contaminated sediments near a petrochemical industry in Gwangyang Bay, Korea, in situ PAH concentrations ranged from 10 to 2,900 ug/kg dry sediment. To enhance the biodegradation rate of PAHs under anaerobic conditions, sediment samples were amended with biostimulating agents alone or in combination: nitrogen and phosphorus in the form of slow-release fertilizer (SRF), lactate, yeast extract (YE), and Tween 80. When added to the sediment individually, all tested agents enhanced the degradation of PAHs, including naphthalene, acenaphthene, anthracene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, and benzo[a]pyrene. Moreover, the combination of SRF, Tween 80, and lactate increased the PAH degradation rate 1.2-8.2 times above that of untreated sediment (0.01-10 ug PAH/kg dry sediment/day). Our results indicated that in situ contaminant PAHs in anoxic sediment, including high molecular weight PAHs, were degraded biologically and that the addition of stimulators increased the biodegradation potential of the intrinsic microbial populations. Our results will contribute to the development of new strategies for in situ treatment of PAH-contaminated anoxic sediments.
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