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Research Support, Non-U.S. Gov'ts
NOTE] Effects of Light Intensity on Components and Topographical Structures of Extracellular Polysaccharides from the Cyanobacteria Nostoc sp.
Hongmei Ge , Ling Xia , Xuping Zhou , Delu Zhang , Chunxiang Hu
J. Microbiol. 2014;52(2):179-183.   Published online February 1, 2014
DOI: https://doi.org/10.1007/s12275-014-2720-5
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AbstractAbstract
A study on the effects of light intensity (40 and 80 μE/m2/sec) on the components and topographical structures of extracellular polysaccharides (EPS) was carried out in cyanobacteria Nostoc sp.. EPS yield increased with light intensity. However, light intensity did not significantly affect the EPS fractions and monosaccharide composition. Higher light intensity generally resulted in higher protein content of EPS in similar fractions. The topographical structure of EPS, investigated by atomic force microscopy, appeared as spherical lumps, chains and networks. The long chains were observed at higher light intensity. Thus, light intensity affected the yield and nature of EPS.

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Predicting the Chemical Composition and Structure of Aspergillus nidulans Hyphal Wall Surface by Atomic Force Microscopy
Hyun-uk Lee , Jong Bae Park , Haeseong Lee , Keon-Sang Chae , Dong-Min Han , Kwang-Yeop Jahng
J. Microbiol. 2010;48(2):243-248.   Published online May 1, 2010
DOI: https://doi.org/10.1007/s12275-010-8094-4
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AbstractAbstract
In fungi, cell wall plays an important role in growth and development. Major macromolecular constituents of the aspergilli cell wall are glucan, chitin, and protein. We examined the chemical composition and structure of the Aspergillus nidulans hyphal wall surface by an atomic force microscope (AFM). To determine the composition of the cell wall surface, the adhesion forces of commercially available β-glucan, chitin, and various proteins were compared to those of corresponding fractions prepared from the hyphal wall. In both setups, the adhesion forces of β-glucan, chitin, and protein were 25-50, 1000-3000, and 125-300 nN, respectively. Adhesion force analysis demonstrated that the cell surface of the apical tip region might contain primarily chitin and β-glucan and relatively a little protein. This analysis also showed the chemical composition of the hyphal surface of the mid-region would be different from that of the apical region. Morphological images obtained by the tapping mode of AFM revealed that the hyphal tip surface has moderate roughness.
Isolation of Listeria monocytogenes by Immunomagnetic Separation and Atomic Force Microscopy
Birce Mercanoglu , S. Aykut Aytac , M. Ali Ergun , Erdal Tan
J. Microbiol. 2003;41(2):144-147.
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AbstractAbstract
Listeria monocytogenes is a pathogen of major concern to the food industry and the potential cause of severe infections such as listeriosis. Early detection of this foodborne pathogen is important in order to eliminate its potential hazards. So, immunomagnetic separation (IMS) has been suggested as a means of reducing the total analysis time and for improving the sensitivity of detection. Atomic force microscopy (AFM) has been used for measuring the topographic properties of sample surfaces at nanometer scale. In this study, we used AFM to confirm both the sensitivity and the specificity of IMS. Regarding AFM analysis, the length and the width of the bacteria, which were in agreement with literature values, were found to be 2.993 mm and 0.837 mm, respectively. As a result, AFM helped us both characterize and measure the bacterial and bead structures.

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