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- [PROTOCOL]A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans
- Kwang-Woo Jung , Kyung-Tae Lee , Yong-Sun Bahn
- J. Microbiol. 2020;58(10):823-831. Published online September 29, 2020
- DOI: https://doi.org/10.1007/s12275-020-0341-8
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- 2 Crossref
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Abstract
-
Signature-tagged mutagenesis (STM) is a high-throughput
genetic technique that can be used to investigate the function
of genes by constructing a large number of mutant strains
with unique DNA identification tags, pooling them, and screening
them for a particular phenotypic trait. STM was first designed
for the identification of genes that contribute to the
virulence or infectivity of a pathogen in its host. Recently, this
method
has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM
method
for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence. -
Citations
Citations to this article as recorded by
- Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
Dayoung Sung, Garam Choi, Minji Ahn, Hokyung Byun, Tae Young Kim, Hojun Lee, Zee-Won Lee, Ji Yong Park, Young Hyun Jung, Ho Jae Han, Sang Ho Choi
Nucleic Acids Research.2024;[Epub] CrossRef - Zinc-binding domain mediates pleiotropic functions of Yvh1 in Cryptococcus neoformans
Jae-Hyung Jin, Myung Kyung Choi, Hyun-Soo Cho, Yong-Sun Bahn
Journal of Microbiology.2021; 59(7): 658. CrossRef
- Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus
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