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- Development of a Novel D‑Lactic Acid Production Platform Based on Lactobacillus saerimneri TBRC 5746
- Kitisak Sansatchanon , Pipat Sudying , Peerada Promdonkoy , Yutthana Kingcha , Wonnop Visessanguan , Sutipa Tanapongpipat , Weerawat Runguphan , Kanokarn Kocharin
- J. Microbiol. 2023;61(9):853-863. Published online September 14, 2023
- DOI: https://doi.org/10.1007/s12275-023-00077-x
- 231 View
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- 5 Web of Science
- 5 Crossref
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Abstract
- D-Lactic acid is a chiral, three-carbon organic acid, that bolsters the thermostability of polylactic acid. In this study, we developed a microbial production platform for the high-titer production of D-lactic acid. We screened 600 isolates of lactic acid bacteria (LAB) and identified twelve strains that exclusively produced D-lactic acid in high titers. Of these strains, Lactobacillus saerimneri TBRC 5746 was selected for further development because of its homofermentative metabolism. We investigated the effects of high temperature and the use of cheap, renewable carbon sources on lactic acid production and observed a titer of 99.4 g/L and a yield of 0.90 g/g glucose (90% of the theoretical yield). However, we also observed L-lactic acid production, which reduced the product’s optical purity. We then used CRISPR/dCas9-assisted transcriptional repression to repress the two Lldh genes in the genome of L. saerimneri TBRC 5746, resulting in a 38% increase in D-lactic acid production and an improvement in optical purity. This is the first demonstration of CRISPR/dCas9-assisted transcriptional repression in this microbial host and represents progress toward efficient microbial production of D-lactic acid.
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Citations
Citations to this article as recorded by
- Converting a D-/L lactic acid bacteria to its d-type counterpart via a combined chemical mutagenesis and biosensor screening method, and its application in lignocellulosic biorefinery
Xingwang Ma, Kang Yan, Yuwei Zhang, Xiaoyu Xie, Shujie Zou, Yuanyuan Sha, Rui Zhai, Zhaoxian Xu, Mingjie Jin
Bioresource Technology.2025; 428: 132471. CrossRef - Industrial–scale production of various bio–commodities by engineered microbial cell factories: Strategies of engineering in microbial robustness
Ju-Hyeong Jung, Vinoth Kumar Ponnusamy, Gopalakrishnan Kumar, Bartłomiej Igliński, Vinod Kumar, Grzegorz Piechota
Chemical Engineering Journal.2024; 502: 157679. CrossRef - Microbial Cell Factories: Biodiversity, Pathway Construction, Robustness, and Industrial Applicability
Rida Chaudhary, Ali Nawaz, Mireille Fouillaud, Laurent Dufossé, Ikram ul Haq, Hamid Mukhtar
Microbiology Research.2024; 15(1): 247. CrossRef - Adaptive Evolution for the Efficient Production of High-Quality d-Lactic Acid Using Engineered Klebsiella pneumoniae
Bo Jiang, Jiezheng Liu, Jingnan Wang, Guang Zhao, Zhe Zhao
Microorganisms.2024; 12(6): 1167. CrossRef - Enhancing D-lactic acid production from non-detoxified corn stover hydrolysate via innovative F127-IEA hydrogel-mediated immobilization of Lactobacillus bulgaricus T15
Yuhan Zheng, Feiyang Sun, Siyi Liu, Gang Wang, Huan Chen, Yongxin Guo, Xiufeng Wang, Maia Lia Escobar Bonora, Sitong Zhang, Yanli Li, Guang Chen
Frontiers in Microbiology.2024;[Epub] CrossRef
- Converting a D-/L lactic acid bacteria to its d-type counterpart via a combined chemical mutagenesis and biosensor screening method, and its application in lignocellulosic biorefinery
- Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber
- Qingqing Yan , Zhouwei Zhang , Yishan Yang , Fusheng Chen , Yanchun Shao
- J. Microbiol. 2018;56(4):255-263. Published online February 28, 2018
- DOI: https://doi.org/10.1007/s12275-018-7425-8
- 178 View
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- 4 Crossref
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Abstract
- Monascus spp. are commonly used for a wide variety of applications in the food and pharmaceutical industries. In previous studies, the knock-out of mrflbA (a putative regulator of the G protein α subunit) in M. ruber led to autolysis of the mycelia, decreased pigmentation and lowered mycotoxin production. Therefore, we aimed to obtain a comprehensive overview of the underlying mechanism of mrflbA deletion at the proteome level. A two-dimensional gel electrophoresis analysis of mycelial proteins indicated that the abundance of 178 proteins was altered in the ΔmrflbA strain, 33 of which were identified with high confidence. The identified proteins are involved in a range of activities, including carbohydrate and amino acid metabolism, hyphal development and the oxidative stress response, protein modification, and the regulation of cell signaling. Consistent with these findings, the activity of antioxidative enzymes and chitinase was elevated in the supernatant of the ΔmrflbA strain. Furthermore, deletion of mrflbA resulted in the transcriptional reduction of secondary metabolites (pigment and mycotoxin). In short, the mutant phenotypes induced by the deletion of mrflbA were consistent with changes in the expression levels of associated proteins, providing direct evidence of the regulatory functions mediated by mrflbA in M. ruber.
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Citations
Citations to this article as recorded by- Histone deacetylase MrHos3 negatively regulates the production of citrinin and pigments in Monascus ruber
Qianrui Liu, Yunfan Zheng, Baixue Liu, Fufang Tang, Yanchun Shao
Journal of Basic Microbiology.2023; 63(10): 1128. CrossRef - Histone deacetylase MrRpd3 plays a major regulational role in the mycotoxin production of Monascus ruber
Yunfan Zheng, Yueyan Huang, Zejing Mao, Yanchun Shao
Food Control.2022; 132: 108457. CrossRef - Characterization of key upstream asexual developmental regulators in Monascus ruber M7
Lili Jia, Yuyun Huang, Jae-Hyuk Yu, Marc Stadler, Yanchun Shao, Wanping Chen, Fusheng Chen
Food Bioscience.2022; 50: 102153. CrossRef - Quantitative Proteomics Analysis by Sequential Window Acquisition of All Theoretical Mass Spectra–Mass Spectrometry Reveals Inhibition Mechanism of Pigments and Citrinin Production of Monascus Response to High Ammonium Chloride Concentration
Bo Zhou, Yifan Ma, Yuan Tian, Jingbo Li, Haiyan Zhong
Journal of Agricultural and Food Chemistry.2020; 68(3): 808. CrossRef
- Histone deacetylase MrHos3 negatively regulates the production of citrinin and pigments in Monascus ruber
Research Support, Non-U.S. Gov't
- The Changes of Proteomes Components of Helicobacter pylori in Response to Acid Stress without Urea
- Chunhong Shao , Qunye Zhang , Wei Tang , Wei Qu , Yabin Zhou , Yundong Sun , Han Yu , Jihui Jia
- J. Microbiol. 2008;46(3):331-337. Published online July 5, 2008
- DOI: https://doi.org/10.1007/s12275-008-0062-x
- 169 View
- 0 Download
- 13 Crossref
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Abstract
- Acid stress is the most obvious challenge Helicobacter pylori encounters in human stomach. The urease system is the basic process used to maintain periplasmic and cytoplasmic pH near neutrality when H. pylori is exposed to acidic condition. However, since the urea concentration in gastric juice is approximately 1 mM, considered possibly insufficient to ensure the survival of H. pylori, it is postulated that additional mechanisms of pH homeostasis may contribute to the acid adaptation in H. pylori. In order to identify the acid-related proteins other than the urease system we have compared the proteome profiles of H. pylori strain 26695 exposed to different levels of external pH (7.4, 6.0, 5.0, 4.0, 3.0, and 2.0) for 30 min in the absence of urea using 2-DE. Differentially expressed proteins were identified by MALDI-TOF-TOF-MS analysis, which turned out to be 36 different proteins. The functions of these proteins included ammonia production, molecular chaperones, energy metabolism, cell envelope, response regulator and some proteins with unknown function. SOM analysis indicated that H. pylori responds to acid stress through multi-mechanisms involving many proteins, which depend on the levels of acidity the cells encounter.
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Citations
Citations to this article as recorded by- Delineation of the pH-Responsive Regulon Controlled by the Helicobacter pylori ArsRS Two-Component System
John T. Loh, Miranda V. Shum, Scott D. R. Jossart, Anne M. Campbell, Neha Sawhney, W. Hayes McDonald, Matthew B. Scholz, Mark S. McClain, Mark H. Forsyth, Timothy L. Cover, Victor J. Torres
Infection and Immunity.2021;[Epub] CrossRef - Proteomic analysis of Rhizobium freirei PRF 81T reveals the key role of central metabolic pathways in acid tolerance
Leandro Datola Tullio, Douglas Fabiano Gomes, Luciano Paulino Silva, Mariangela Hungria, Jesiane Stefania da Silva Batista
Applied Soil Ecology.2019; 135: 98. CrossRef - Acid‐regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization
Elizabeth A. Marcus, George Sachs, David R. Scott
Helicobacter.2018;[Epub] CrossRef - The histone‐like protein HU has a role in gene expression during the acid adaptation response in Helicobacter pylori
Alhejandra Álvarez, Héctor Toledo
Helicobacter.2017;[Epub] CrossRef - Stable isotope labeling by amino acids in cell culture based proteomics reveals differences in protein abundances between spiral and coccoid forms of the gastric pathogen Helicobacter pylori
Stephan A. Müller, Sandy R. Pernitzsch, Sven-Bastiaan Haange, Peter Uetz, Martin von Bergen, Cynthia M. Sharma, Stefan Kalkhof
Journal of Proteomics.2015; 126: 34. CrossRef - Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori
Manuel Valenzuela, Aníbal Cáceres, Oscar Almarza, Denisse Bravo, Sarita Soto, Oscar Cerda, Héctor Toledo
Helicobacter.2014; 19(3): 182. CrossRef - Identification of new protein coding sequences and signal peptidase cleavage sites of Helicobacter pylori strain 26695 by proteogenomics
Stephan A. Müller, Sven Findeiß, Sandy R. Pernitzsch, Dirk K. Wissenbach, Peter F. Stadler, Ivo L. Hofacker, Martin von Bergen, Stefan Kalkhof
Journal of Proteomics.2013; 86: 27. CrossRef - Effect of gastric environment on Helicobacter pylori adhesion to a mucoadhesive polymer
Frederico Nogueira, Inês C. Gonçalves, M. Cristina L. Martins
Acta Biomaterialia.2013; 9(2): 5208. CrossRef - Analysis of Aztreonam-Inducing Proteome Changes in Nondividing Filamentous Helicobacter pylori
Chunhong Shao, Yabin Zhou, Yundong Sun, Hongyan Wang, Wei Qu, Han Yu, Chunyan Chen, Jihui Jia
Current Microbiology.2012; 65(1): 108. CrossRef - Identification of S-nitrosylation of proteins of Helicobacter pylori in response to nitric oxide stress
Wei Qu, Yabin Zhou, Yundong Sun, Ming Fang, Han Yu, Wenjuan Li, Zhifang Liu, Jiping Zeng, Chunyan Chen, Chengjiang Gao, Jihui Jia
The Journal of Microbiology.2011; 49(2): 251. CrossRef - Virulence factor cytotoxin-associated gene A in Helicobacter pylori is downregulated by interferon-γin vitro
Yinghui Zhao, Yabin Zhou, Yundong Sun, Ailian Yu, Han Yu, Wenjuan Li, Zhifang Liu, Jiping Zeng, Xi Li, Chunyan Chen, Jihui Jia
FEMS Immunology & Medical Microbiology.2011; 61(1): 76. CrossRef - Functional and structural aspects of helicobacter pylori acidic stress response factors
Giuseppe Zanotti, Laura Cendron
IUBMB Life.2010; 62(10): 715. CrossRef - Helicobacter pylori proteins response to nitric oxide stress
Wei Qu, Yabin Zhou, Chunhong Shao, Yundong Sun, Qunye Zhang, Chunyan Chen, Jihui Jia
The Journal of Microbiology.2009; 47(4): 486. CrossRef
- Delineation of the pH-Responsive Regulon Controlled by the Helicobacter pylori ArsRS Two-Component System
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