MSK
Search
- Page Path
- HOME > Search
Research Support, Non-U.S. Gov't
- Use of Denaturing High-Performance Liquid Chromatography (DHPLC) to Characterize the Bacterial and Fungal Airway Microbiota of Cystic Fibrosis Patients
- Jérôme Mounier , Audrey Gouëllo , Marlène Keravec , Solène Le Gal , Grégory Pacini , Stella Debaets , Gilles Nevez , Gilles Rault , Georges Barbier , Geneviève Héry-Arnaud
- J. Microbiol. 2014;52(4):307-314. Published online February 17, 2014
- DOI: https://doi.org/10.1007/s12275-014-3425-5
- 49 View
- 0 Download
- 8 Crossref
-
Abstract
- The aim of this study was to evaluate the use of denaturing high-performance liquid chromatography (DHPLC) to characterize cystic fibrosis (CF) airway microbiota including both bacteria and fungi. DHPLC conditions were first optimized using a mixture of V6, V7 and V8 region 16S rRNA gene PCR amplicons from 18 bacterial species commonly found in CF patients. Then, the microbial diversity of 4 sputum samples from 4 CF patients was analyzed using cultural methods, cloning/sequencing (for bacteria only) and DHPLC peak fraction collection/sequencing. DHPLC analysis allowed identifying more bacterial and fungal species than the classical culture methods, including well-recognized pathogens such as Pseudomonas aeruginosa. Even if a lower number of bacterial Operational Taxonomic Units (OTUs) was identified by DHPLC, it allowed to find OTUs unidentified by cloning/sequencing. The combination of both techniques permitted to correlate the majority of DHPLC peaks to defined OTUs. Finally, although Aspergillus fumigatus detection using DHPLC can still be improved, this technique clearly allowed to identify a higher number of fungal species versus classical culture-based methods. To conclude, DHPLC provided meaningful additional data concerning pathogenic bacteria and fungi as well as fastidious microorganisms present within the CF respiratory tract. DHPLC can be considered as a complementary technique to culture-dependent analyses in routine microbiological laboratories.
-
Citations
Citations to this article as recorded by
- Chronic Diseases Associated with Malassezia Yeast
Abdourahim Abdillah, Stéphane Ranque
Journal of Fungi.2021; 7(10): 855. CrossRef - The Mycobiome in Health and Disease: Emerging Concepts, Methodologies and Challenges
Pei Yee Tiew, Micheál Mac Aogain, Nur A’tikah Binte Mohamed Ali, Kai Xian Thng, Karlyn Goh, Kenny J. X. Lau, Sanjay H. Chotirmall
Mycopathologia.2020;[Epub] CrossRef - Developing collaborative works for faster progress on fungal respiratory infections in cystic fibrosis
Carsten Schwarz, Patrick Vandeputte, Amandine Rougeron, Sandrine Giraud, Thomas Dugé de Bernonville, Ludovic Duvaux, Amandine Gastebois, Ana Alastruey-Izquierdo, Maria Teresa Martín-Gomez, Estrella Martin Mazuelos, Amparo Sole, Josep Cano, Javier Pemán, G
Medical Mycology.2018; 56(suppl_1): S42. CrossRef -
Aspergillus Species in Bronchiectasis: Challenges in the Cystic Fibrosis and Non-cystic Fibrosis Airways
Sanjay H. Chotirmall, Maria Teresa Martin-Gomez
Mycopathologia.2018; 183(1): 45. CrossRef - Fusarium Profiling and Barley Malt Gushing Propensity
Vertti Virkajärvi, Tuija Sarlin, Arja Laitila
Journal of the American Society of Brewing Chemists.2017; 75(3): 181. CrossRef - The lung mycobiome: an emerging field of the human respiratory microbiome
Linh D. N. Nguyen, Eric Viscogliosi, Laurence Delhaes
Frontiers in Microbiology.2015;[Epub] CrossRef - The Airway Colonization by Opportunistic Filamentous Fungi in Patients with Cystic Fibrosis: Recent Updates
Kada Touati, Do Ngoc Linh Nguyen, Laurence Delhaes
Current Fungal Infection Reports.2014; 8(4): 302. CrossRef - Characterization and quantification of the fungal microbiome in serial samples from individuals with cystic fibrosis
Sven D Willger, Sharon L Grim, Emily L Dolben, Anna Shipunova, Thomas H Hampton, Hilary G Morrison, Laura M Filkins, George A O‘Toole, Lisa A Moulton, Alix Ashare, Mitchell L Sogin, Deborah A Hogan
Microbiome.2014;[Epub] CrossRef
- Chronic Diseases Associated with Malassezia Yeast
Journal Article
- Burkholderia cepacia Complex Infection in a Cohort of Italian Patients with Cystic Fibrosis
- Antonietta Lambiase , Valeria Raia , Stefania Stefani , Angela Sepe , Pasqualina Ferri , Paolo Buonpensiero , Fabio Rossano , Mariassunta Del Pezzo
- J. Microbiol. 2007;45(3):275-279.
- DOI: https://doi.org/2530 [pii]
- 37 View
- 0 Download
-
Abstract
- The aims of this study were to detect Burkholderia cepacia complex (Bcc) strains in a cohort of Cystic Fibrosis patients (n=276) and to characterize Bcc isolates by molecular techniques. The results showed that 11.23% of patients were infected by Bcc. Burkholderia cenocepacia lineage III-A was the most prevalent species (64.3%) and, of these, 10% was cblA positive and 50% esmR positive. Less than half of the strains were sensitive to ceftazidime, meropenem, piperacillin tazobactam, and trimethoprim-sulfamethoxazole. About half of the strains (41%) had homogeneous profiles, suggesting cross-transmission. The infection by B. cenocepacia was associated to a high rate of mortality (p=0.01).
First
Prev
TOP
