Research Support, Non-U.S. Gov't
- Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau
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Huili Pang , Zhongfang Tan , Guangyong Qin , Yanping Wang , Zongwei Li , Qingsheng Jin , Yimin Cai
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J. Microbiol. 2012;50(1):63-71. Published online February 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1284-5
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Abstract
- A total of 140 lactic acid bacteria (LAB) strains were isolated
from corn, alfalfa, clover, sainfoin, and Indian goosegrass
in the Tibetan Plateau. According to phenotypic and chemotaxonomic
characteristics, 16S rDNA sequence, and recA
gene PCR amplification, these LAB isolates were identified
as belonging to five genera and nine species. Corn contained
more LAB species than other forage crops. Leuconostoc
pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus
brevis, and Weissella paramesenteroides were dominant
members of the LAB population on alfalfa, clover, sainfoin,
and Indian goosegrass, respectively. The comprehensive
16S rDNA and recA-based approach effectively described
the LAB community structure of the relatively abundant LAB
species distributed on different forage crops. This is the
first report describing the diversity and natural populations
of LAB associated with Tibetan forage crops, and most isolates
grow well at or below 10°C. The results will be valuable for
the future design of appropriate inoculants for silage fermentation
in this very cold area.