Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau

Huili Pang; Zhongfang Tan; Guangyong Qin; Yanping Wang; Zongwei Li; Qingsheng Jin; Yimin Cai

doi:10.1007/s12275-012-1284-5

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Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau: Huili Pang ¹, Zhongfang Tan ¹, Guangyong Qin ¹, Yanping Wang ¹, Zongwei Li ¹, Qingsheng Jin ², Yimin Cai ³; Journal of Microbiology 2012;50(1):63-71
DOI: https://doi.org/10.1007/s12275-012-1284-5
Published online: February 27, 2012

¹Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, 450052 Zhengzhou, Henan, P. R. China, ²Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, Zhejiang, P. R. China, ³Japan International Research Center for Agricultural Sciences, 305-8686 Tsukuba, Ibaraki, Japan¹Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, 450052 Zhengzhou, Henan, P. R. China, ²Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, Zhejiang, P. R. China, ³Japan International Research Center for Agricultural Sciences, 305-8686 Tsukuba, Ibaraki, Japan

Corresponding author: Yimin Cai , Tel: +81-29-838-6365,

Received: 7 June 2011 • Accepted: 31 October 2011

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Abstract

A total of 140 lactic acid bacteria (LAB) strains were isolated from corn, alfalfa, clover, sainfoin, and Indian goosegrass in the Tibetan Plateau. According to phenotypic and chemotaxonomic characteristics, 16S rDNA sequence, and recA gene PCR amplification, these LAB isolates were identified as belonging to five genera and nine species. Corn contained more LAB species than other forage crops. Leuconostoc pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus brevis, and Weissella paramesenteroides were dominant members of the LAB population on alfalfa, clover, sainfoin, and Indian goosegrass, respectively. The comprehensive 16S rDNA and recA-based approach effectively described the LAB community structure of the relatively abundant LAB species distributed on different forage crops. This is the first report describing the diversity and natural populations of LAB associated with Tibetan forage crops, and most isolates grow well at or below 10°C. The results will be valuable for the future design of appropriate inoculants for silage fermentation in this very cold area.

Keywords: forage; epiphytic microflora; lactic acid bacteria; 16S rDNA; recA gene

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Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau

J. Microbiol. 2012;50(1):63-71. Published online February 27, 2012

DOI: https://doi.org/10.1007/s12275-012-1284-5

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