Microflora Profiling of Infected Root Canal before and after Treatment Using Culture-Independent Methods

Yasuhiro Ito; Takuichi Sato; Keiko Yamaki; Gen Mayanagi; Kazuhiro Hashimoto; Hidetoshi Shimauchi; Nobuhiro Takahashi

doi:10.1007/s12275-012-0459-4

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Microflora Profiling of Infected Root Canal before and after Treatment Using Culture-Independent Methods: Yasuhiro Ito ^1,2, Takuichi Sato ², Keiko Yamaki ¹, Gen Mayanagi ¹, Kazuhiro Hashimoto ¹, Hidetoshi Shimauchi ¹, Nobuhiro Takahashi ²; Journal of Microbiology 2012;50(1):58-62
DOI: https://doi.org/10.1007/s12275-012-0459-4
Published online: February 27, 2012

¹Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan, ²Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan¹Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan, ²Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan

Corresponding author: Takuichi Sato , Tel: +81-22-717-8295,

Received: 10 November 2010 • Accepted: 12 October 2011

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Abstract

This study aimed to profile the microflora in infected root canals before and after root canal treatment using cultureindependent
methods
. Six infected root canals in singlerooted teeth with periapical lesions from five subjects were included. Quantification of total bacteria was performed by real-time PCR with primers targeting 16S rRNA genes. PCR products with universal 16S rRNA gene primers were cloned and partially sequenced, and bacterial identification at the species level was performed by comparative analysis with the GenBank database. The concentration of extracted DNA before treatment was higher than that after root canal treatment, although the difference was not statistically significant. Sequence analysis revealed that oral bacteria such as Fusobacterium, Streptococcus, Olsenella, and Pseudoramibacter detected in cases before root canal treatment disappeared after treatment. These results suggest that the root canal microflora are distinct before and after root canal treatment, and that treatment changes the microflora in both quantity and quality.

Keywords: 16S ribosomal RNA; microflora; PCR; root canals

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Microflora Profiling of Infected Root Canal before and after Treatment Using Culture-Independent Methods

J. Microbiol. 2012;50(1):58-62. Published online February 27, 2012

DOI: https://doi.org/10.1007/s12275-012-0459-4

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