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Purification and Characterization of a Novel Laccase from the Edible Mushroom Hericium coralloides
Ya-Jie Zou 1, He-Xiang Wang 2, Tzi-Bun Ng 3, Chen-Yang Huang 1, Jin-Xia Zhang 1
Journal of Microbiology 2012;50(1):72-78.
DOI: https://doi.org/10.1007/s12275-012-1372-6
Published online: February 27, 2012
1Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China, 2State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China, 3Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China
Corresponding author:  Jin-Xia Zhang , Tel: +86-10-8210-6207, 
Received: 29 July 2011   • Accepted: 3 August 2011
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Abstract

A novel laccase from the edible mushroom Hericium coralloides was purified by ion exchange chromatography on diethylaminoethyl (DEAE) cellulose, carboxymethyl (CM) cellulose, and Q-Sepharose columns followed by fast protein liquid chromatography gel filtration on a Superdex 75 column. Analysis by gel filtration and SDS-PAGE indicated that the protein is a monomer in solution with a molecular mass of 65 kDa. Its N-terminal amino acid sequence was AVGDDTPQLY, which exhibits partial sequence homology to previously isolated laccases. Optimum activity was observed at pH 2.2 and at 40°C. The enzyme showed activity toward a variety of substrates, the most sensitive of which was 2,2􍿁-azinobis [3-ethylbenzothiazolone-6-sulfonic acid] diammonium salt (ABTS). The degradation activity toward substrates was ABTS > N,N-dimethyl-1,4-phenylenediamine > catechol > 2-methylcatechol > pyrogallol. The laccase did not exert any antiproliferative activity against Hep G2 or MCF 7 tumor cell lines at a concentration of 60 μM, unlike some previously reported mushroom proteins, but showed significant activity toward human immunodeficiency virus-1 (HIV-1) reverse transcriptase with an IC50 of 0.06 μM.

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    Purification and Characterization of a Novel Laccase from the Edible Mushroom Hericium coralloides
    J. Microbiol. 2012;50(1):72-78.   Published online February 27, 2012
    DOI: https://doi.org/10.1007/s12275-012-1372-6
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