Journal of Microbiology

Electrophoretic Karyotypes of Fusarium oxysporum formae speciales: Min, Byung Re , Kim, Kyung Ah , Choi, Yong Keel; J. Microbiol. 1998;36(1):14-19.

Abstract: Chromosomal DNAs from nine strains of Fusarium oxysporum formae speciales were analysed by pulsed field gel electrophoresis (PFGE). Intact chromosomal DNA were prepared from fungal protoplasts. About 7-13 chromosomal DNA bands were separated in nine Fusarium oxysporum formaespeciales, ranging in size from 0.8 to 6.7 Mb. Considering that some of the chromosomal bands represented unresolved doublets, the total genome size was estimated to vary between at least 22.3 and 45.5 Mb. Gels run under different conditions were Southern blotted and probed with randomly selected genomic DNA from Fusarium oxysporum f. sp. lilii. Electrophoretix karyotyping revealed some differences within and between Fusarium oxysporum formae speciales.

Variation of the Intergenic Spacer (IGS) Region of Ribosomal DNA among Fusarium oxysporum formae speciales: Hyun-Jung Kim , Yong-Keel Choi , Byung-Re Min; J. Microbiol. 2001;39(4):265-272.

Abstract: Variation within the intergenic spacer (IGS) of the ribosomal DNA gene for twenty-two strains of F. oxysporum and its formae speciales was examined by PCR, coupled with RFLP analysis. The length of the amplified IGS region was about 2.6 kb in all strains except F. oxysporum f. sp. cucumerinum from Korea and F. oxysporum f. sp. niveum. Those two strains were 2.5 kb long. Restriction digestion of IGS-RFLP regions by EcoRI, NruI, HincII, SalI, SmaI, BglII, HindIII, XhoI, and KpnI gave rise to nine IGS haplotypes among all strains. Cluster analysis based on the presence or absence of comigrating restriction fragments show the two groups based on 44% genetic similarity. These results demonstrated that analysis of IGS showed some difference within and between F. oxysporum formae speciales.

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