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- Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus
- Jae Woong Lee , Yu-Jung Won , Lae Hyung Kang , Sung-Geun Lee , Seung-Won Park , Soon-Young Paik
- J. Microbiol. 2020;58(8):711-715. Published online May 18, 2020
- DOI: https://doi.org/10.1007/s12275-020-0109-1
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- 9 Web of Science
- 9 Crossref
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Abstract
-
Severe fever with thrombocytopenia syndrome (SFTS) is being
reported annually in South Korea since its first detection
there in 2010. The causal agent is a negative-strand RNA
virus 80–100 nm in diameter. It causes fever, thrombocytopenia,
leukocytopenia, gastrointestinal symptoms, and neural
symptoms. The mortality rate of SFTS was 32.6% among 172
case
s reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RTLAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS. -
Citations
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