Journal of Microbiology

Human Antibody Responses to Capsular Polysaccharides of Streptococcus pneumoniae 6B, 14, and 19F: Kim, Ji Hye , Kim, Kyung Hyo , Kim, Jung Soo , Song, Jae Ho , Park, Moon Kook; J. Microbiol. 1998;36(4):303-307.

Abstract: Human antibody responses to Streptococcus pneumoniae 6B, 14, and 19F capsular polysaccharide were analyzed. Thirty-one healthy young adults were immunized with the pneumococcal 23-valent PS vaccine. serum samples were obtained from them before and 1 month after vaccination. The amounts of total antibody, heavy chain and light chain isotypes were determined by enzyme-linked immunosorbent assay (ELISA). Vaccination increased the total lebvels of anti6B, anti-14, and anti-19F PS antibodies by 3.4-fold, 3.8-fold and 4.1-fold, respectively. Some inantibody was predominant in the responses to the three PSs, and most of the IgG anti-PS antibodies were IgG2 isotype. There was no significant difference in the k and λresponses.

Generation of Isotype Switch Variants form Hybridoma cells Producing anti-Streptococcus penumoniae 6B Polysaccharide Antibody: Kim, Ji Hye , Ryu, Eun Ja , Park, Moon Kook; J. Microbiol. 1999;37(3):180-184.

Abstract: Hybridoma cells producing IgM anti-pneuococcal 6B polysaccharide antibodies were induced to switch to IgG-producing cells in vitro by treating with acridine orange. Treating 0.5 ㎍/ml of acridine orange for 24 hours generated maximal number of variant cells. The maximal isotype switch frequency was 3×10^-5, which is about 30-fold higher than the frequency of spontaneous switching. Resulting IgG-producing variants were enriched by sib selection and ELISA spot assay. Two IgG3-producing variant cells were finally cloned by limiting dilution. The variant cells produced similar amounts of antibodies as their parental cells did. The two switched antibodies had similar reactivity to pneumococcal 6B polysaccharide. When compared to their parental IgM antibodies, the switched IgG3 than that of IgM antibody. The antibodies will be useful as essential tools for comparative study of the role of heavy chain isotypes in protection against Streptococcus pneumoniae.

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