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- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
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Special Issues
- Pioneering strategies for overcoming bacterial drug resistance (2026)
- Advancing microbial engineering through synthetic biology (2025)
- Host-associated microbiome (2024)
- Bacterial regulatory mechanisms for the control of complex cellular mechanisms (2023)
- Two years into COVID-19 pandemic: Where are we? (2022)
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- Berberine promotes host lipolysis to enhance antimicrobial defense against hypervirulent Klebsiella pneumoniae infection
- Ju Yeong Lee, Hui-Jung Jung, Anwesha Ash, Seunghyeon Jeon, Miri Hyun, Ji Yeon Lee, Sang-Hee Lee, Hyuk Nam Kwon, Won-Ki Baek, Jichan Jang, Hyun ah Kim, Jin Kyung Kim
- J. Microbiol. 2026;64(8):e2605001. Published online August 31, 2026
- DOI: https://doi.org/10.71150/jm.2605001
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Abstract
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Supplementary Material -
Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen that causes severe community-acquired infections; however, the immune mechanisms controlling intracellular hvKp have yet to be clearly defined. In this study, we investigated the therapeutic potential of berberine against hvKp infection and elucidated the underlying molecular mechanisms using macrophage cell models and in vivo zebrafish models. Berberine significantly reduced intracellular hvKp survival in macrophages and improved survival in hvKp-infected zebrafish. Berberine markedly attenuated proinflammatory cytokine production and inhibited the c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) signaling pathways. Notably, we found that hvKp exploited host lipid droplets (LD) biosynthesis to support its intracellular survival, and berberine effectively suppressed LD accumulation. Mechanistically, berberine promoted the nuclear translocation of transcription factor EB (TFEB), thereby enhancing lipolysis. Although berberine upregulated autophagy-related gene expression during hvKp infection, it did not induce lipophagy, the selective autophagic degradation of LD. Collectively, these findings indicate that berberine has potential as a therapeutic agent against hvKp infection by modulating host lipid metabolism to restrict bacterial intracellular survival.
- Potential of marine dinoflagellates Amphidinium carterae and Prorocentrum minimum as fatty acids producers: A comparative transcriptome and fatty acid profiling study
- Han-Sol Kim, Su-Hwa Lee, Zhun Li, Hah Young Yoo, Jang-Seu Ki
- J. Microbiol. 2026;64(6):e2604005. Published online June 30, 2026
- DOI: https://doi.org/10.71150/jm.2604005
- 792 View
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Marine dinoflagellates are gaining attention as sustainable bioresource for polyunsaturated fatty acids (PUFAs), particularly omega-3 such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In the present study, we analyzed the FAs and transcriptomic profiles of marine dinoflagellates Amphidinium carterae (D-044) and Prorocentrum minimum (D-127) to evaluate their potential as FAs producers. Gas chromatography-FA methyl ester (GC-FAME) analysis showed that A. carterae is a superior omega-3 producer, yielding a total FA content of 67.6 mg/g DW. DHA accounted for 26.7% of the total FAME profile, which is significantly higher than that of P. minimum (18.1 mg/g DW; DHA 13.1%). Gene Ontology (GO) annotation revealed genes related to FAs and lipid metabolism in A. carterae (1,217 genes) and in P. minimum (2,317 genes), which provide a molecular basis for dinoflagellates with high lipid productivity. Notably, three lipid droplet-associated hydrolase (LDAH) genes with diverse evolutionary origins were identified from A. carterae. These findings suggest a potential expansion of the genetic repertoire related to lipid storage and metabolism, highlighting A. carterae and LDAH as candidates for future biotechnological applications and microalgal metabolic engineering.
- The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe
- Alex Meyers , Karuna Chourey , Taylor M. Weiskittel , Susan Pfiffner , John R. Dunlap , Robert L. Hettich , Paul Dalhaimer
- J. Microbiol. 2017;55(2):112-122. Published online January 26, 2017
- DOI: https://doi.org/10.1007/s12275-017-6205-1
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- 16 Crossref
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Abstract
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- Lipid droplets consist of a core of neutral lipids surrounded by a phospholipid monolayer with bound proteins. Much of the information on lipid droplet function comes from proteomic and lipodomic studies that identify the components of droplets isolated from organisms throughout the phylogenetic tree. Here, we add to that important inventory by reporting lipid droplet factors from the fission yeast, Schizosaccharomyces pombe. Unique to this study was the fact that cells were cultured in three different environments: 1) late log growth phase in glucose-based media, 2) stationary phase in glucosebased media, and 3) late log growth phase in media containing oleic acid. We confirmed colocalization of major factors with lipid droplets using live-cell fluorescent microscopy. We also analyzed droplets from each of the three conditions for sterol ester (SE) and triacylglycerol (TAG) content, along with their respective fatty acid compositions. We identified a previously undiscovered lipid droplet protein, Vip1p, which affects droplet size distribution. The results provide further insight into the workings of these ubiquitous organelles.
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Citations
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Anna Marešová, Michaela Grulyová, Miluše Hradilová, Viacheslav Zemlianski, Jarmila Princová, Martin Převorovský, Cathy Savage-Dunn
PLOS Genetics.2024; 20(12): e1011509. CrossRef - Mild Heat Stress Alters the Physical State and Structure of Membranes in Triacylglycerol-Deficient Fission Yeast, Schizosaccharomyces pombe
Péter Gudmann, Imre Gombos, Mária Péter, Gábor Balogh, Zsolt Török, László Vígh, Attila Glatz
Cells.2024; 13(18): 1543. CrossRef - Lipid droplets: a cellular organelle vital in cancer cells
Yi Jin, Yanjie Tan, Jian Wu, Zhuqing Ren
Cell Death Discovery.2023;[Epub] CrossRef - Oleaginous yeasts: Time to rethink the definition?
José Manuel Salvador López, Meriam Vandeputte, Inge N. A. Van Bogaert
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Molecular Biology of the Cell.2022;[Epub] CrossRef - Lipid Droplet Nucleation
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Trends in Cell Biology.2021; 31(2): 108. CrossRef - Metabolism of Storage Lipids and the Role of Lipid Droplets in the Yeast Schizosaccharomyces pombe
Ivan Hapala, Peter Griac, Roman Holic
Lipids.2020; 55(5): 513. CrossRef - Lipid Droplets in Neurodegenerative Disorders
Brandon C. Farmer, Adeline E. Walsh, Jude C. Kluemper, Lance A. Johnson
Frontiers in Neuroscience.2020;[Epub] CrossRef - Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts
Neha Arora, Hong-Wei Yen, George P. Philippidis
Sustainability.2020; 12(12): 5125. CrossRef - Mechanisms of protein targeting to lipid droplets: A unified cell biological and biophysical perspective
Ravi Dhiman, Stefanie Caesar, Abdou Rachid Thiam, Bianca Schrul
Seminars in Cell & Developmental Biology.2020; 108: 4. CrossRef - The New Face of the Lipid Droplet: Lipid Droplet Proteins
Congyan Zhang, Pingsheng Liu
PROTEOMICS.2019;[Epub] CrossRef - Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells
Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Anna M. Kot
Biological Trace Element Research.2019; 187(1): 316. CrossRef - The Peroxygenase Activity of the Aspergillus flavus Caleosin, AfPXG, Modulates the Biosynthesis of Aflatoxins and Their Trafficking and Extracellular Secretion via Lipid Droplets
Abdulsamie Hanano, Mari Alkara, Ibrahem Almousally, Mouhnad Shaban, Farzana Rahman, Mehedi Hassan, Denis J. Murphy
Frontiers in Microbiology.2018;[Epub] CrossRef - Mitotic defects in fission yeast lipid metabolism ‘cut’ mutants are suppressed by ammonium chloride
Róbert Zach, Jarmila Tvarůžková, Martin Schätz, Ondřej Ťupa, Beáta Grallert, Martin Převorovský
FEMS Yeast Research.2018;[Epub] CrossRef - Lipid Droplets: Formation to Breakdown
Alex Meyers, Taylor M. Weiskittel, Paul Dalhaimer
Lipids.2017; 52(6): 465. CrossRef
- Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast
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