Journal of Microbiology

Research Support, Non-U.S. Gov'ts

Antiviral Effect of Flavonol Glycosides Isolated from the Leaf of Zanthoxylum piperitum on Influenza Virus: Song-Yi Ha , Hana Youn , Chang-Seon Song , Se Chan Kang , Jong Jin Bae , Hee Tae Kim , Kwang Min Lee , Tae Hoon Eom , In Su Kim , Jong Hwan Kwak; J. Microbiol. 2014;52(4):340-344. Published online March 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4073-5

Abstract: The ethanol extract of Zanthoxylum piperitum (L.) DC. showed in vitro antiviral activity against influenza A virus. Three flavonol glycosides were isolated from the EtOAc fraction of Z. piperitum leaf by means of activity-guided chromatographic separation. Structures of isolated compounds were identified as quercetin 3-O-β-D-galactopyranoside (1), quercetin 3-O-α-L-rhamnopyranoside (2), kaempferol 3-O-α-L-rhamnopyranoside (3) by comparing their spectral data with literature values. The anti-influenza viral activity of isolates was evaluated using a plaque reduction assay against influenza A/NWS/33 (H1N1) virus. The compounds also were subjected to neuraminidase inhibition assay in influenza A/NWS/33 virus. Compounds 1-3 exhibited antiviral activity against an influenza A virus in vitro, and inhibited the neuraminidase activity at relatively high concentrations.

NOTE] Susceptibility of Human H3N2 Influenza Virus to Oseltamivir in South Korea, 2009–2011: Sehee Park , Jin Il Kim , Ilseob Lee , Sangmoo Lee , Min-Woong Hwang , Joon-Yong Bae , Jun Heo , Eun-Joo Lim , Won-Seok Seok , Hee Jin Cheong , Joon Young Song , Woo Joo Kim , Man-Seong Park; J. Microbiol. 2012;50(6):1067-1070. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2541-3

Abstract: During the 2009–2011 influenza seasons, 10.26% of the specimens isolated from patients in South Korea were subtyped as H3N2 viruses. Some oseltamivir-sensitive H3N2 samples exhibited different plaque morphologies, and were found to have novel mutations in the neuraminidase gene. In a subsequent analysis using NA mutant viruses, viral compensation against oseltamivir treatment was observed only in the N2 mutant virus. All things considered, these novel mutations may account for the exclusive characteristics of selected H3N2 viruses observed in plaque reduction assays.

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