Journal of Microbiology

Production of lipocortin-1_1-185 using a recombinant of escherichia coli: Lee, Kyung Il , Oh, Kyung Hee , Lee, Jung Hyun , Na, Do Sun , Lee, Kye Joon; J. Microbiol. 1997;35(2):123-126.

Abstract: The aim of the present study was to optimize culture condition for the expression of lipocortin 1_1-185 in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1_1-185 gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28℃ for seed culture and 40℃ for main culture and the optimum pH was 7.0. The production of Lipocortin-1_1-185 was closely associated with cell growth and related to plasmid amplification.

Search