Journal of Microbiology

Journal Article

Amino acid residues in the Ler protein critical for derepression of the LEE5 promoter in enteropathogenic E. coli: Su-Mi Choi , Jae-Ho Jeong , Hyon E. Choy , Minsang Shin; J. Microbiol. 2016;54(8):559-564. Published online August 2, 2016; DOI: https://doi.org/10.1007/s12275-016-6027-6

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Enteropathogenic E. coli causes attaching and effacing (A/E) intestinal lesions. The genes involved in the formation of A/E lesions are encoded within a chromosomal island comprising of five major operons, LEE1-5. The global regulator H-NS represses the expression of these operons. Ler, a H-NS homologue, counteracts the H-NS–mediated repression. Using a novel genetic approach, we identified the amino acid residues in Ler that are involved in the interaction with H-NS: I20 and L23 in the C-terminal portion of α-helix 3, and I42 in the following unstructured linker region.

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Regulation of the Locus of Enterocyte Effacement in Attaching and Effacing Pathogens
R. Christopher D. Furniss, Abigail Clements, William Margolin
Journal of Bacteriology.2018;[Epub] CrossRef

Research Support, Non-U.S. Gov'ts

Epidemiological Investigation of eaeA-Positive Escherichia coli and Escherichia albertii Strains Isolated from Healthy Wild Birds: Jae-Young Oh , Min-Su Kang , Hee-Tae Hwang , Byung-Ki An , Jun-Hun Kwon , Yong-Kuk Kwon; J. Microbiol. 2011;49(5):747-752. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-1133-y

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Abstract: Escherichia coli has commonly been associated with diarrheal illness in humans and animals. Recently, E. albertii has been reported to be a potential pathogen of humans and animals and to be carried by wild birds. In the present study, the prevalence and genetic characteristics of intimin-producing E. coli and E. albertii strains were evaluated in wild birds in Korea. Thirty one of 790 Enterobacteriaceae strains from healthy wild birds were positive for the intimin gene (eaeA) and twenty two of the 31 strains were identified as atypical enteropathogenic E. coli (aEPEC) that did not possess both EAF and bfpA genes. A total of nine lactose non-fermenting coliform bacterial strains were identified as E. albertii by PCR and sequence analysis of housekeeping genes. A total of 28 (90.3%) eaeA-positive strains were isolated from waterfowl. Fifteen aEPEC (68.2%) and two E. albertii (22.2%) strains had a β-intimin subtype and 14 aEPEC strains harboring β-intimin belonged to phylogenetic group B2. All eaeA-positive E. albertii and 3 aEPEC strains possessed the cytolethal distending toxin gene (cdtB). The eaeA-positive E. coli and E. albertii strains isolated from healthy wild birds need to be recognized as a potential pathogroup that may pose a potential threat to human and animal health. These findings indicate that eaeA-positive E. coli as well as E. albertii can be carried by wild birds, posing a potential threat to human and animal health.

Enteric Bacteria Isolated from Acute Diarrheal Patients in the Republic of Korea between the Year 2004 and 2006: Seung-Hak Cho , Hyun-Ho Shin , Yeon-Hwa Choi , Mi-Sun Park , Bok-Kwon Lee; J. Microbiol. 2008;46(3):325-330. Published online July 5, 2008; DOI: https://doi.org/10.1007/s12275-008-0015-4

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Abstract: In an epidemiological survey of human enterobacterial infections in the Republic of Korea during three years from 2004 to 2006, we isolated 1,784 (6.2%, isolation rate of enteropathogens from stool samples) in 2004, 2,547 (9.5%) in 2005 and 3,506 bacteria (12.3%) from people who visited clinics. Among the isolated bacteria, pathogenic Escherichia coli, especially, EAEC was the most frequently identified pathogen in both urban and rural regions followed by Staphylococcus aureus, Salmonella species, Bacillus cereus, Vibrio parahaemolyticus, Campylobacter jejuni, Clostridium perfringens, and Shigella species. Distinct seasonality was found in V. parahaemolyticus species, while this pathogen showed no age-specific patterns. However, other bacteria, i.e., pathogenic E. coli, S. aureus, Salmonella spp., and B. cereus showed similar seasonality throughout the year, showing a slight increase in the infection rate during the summer months and high prevalence among children under 10 years of age and elder-age people. The antibiotic susceptibility patterns of pathogenic E. coli, Salmonella spp., and S. aureus showed high resistance to penicillins. However, both pathogenic E. coli and Salmonella spp. were susceptible to several cephems, imipenem, and amikacin. Moreover, S. aureus strains resistant to vancomycin were not found. In conclusion, these surveillances can play an important role for the control and prevention to the diseases originated by enteritis bacteria.

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