MSK
Search
- Page Path
- HOME > Search
Full article
- PhoU interaction with the PhoR PAS domain is required for repression of the pho regulon and Salmonella virulence, but not for polyphosphate accumulation
- Seungwoo Baek, Soomin Choi, Yoontak Han, Eunna Choi, Shinae Park, Jung-Shin Lee, Eun-Jin Lee
- J. Microbiol. 2025;63(9):e2505013. Published online September 30, 2025
- DOI: https://doi.org/10.71150/jm.2505013
- 1,027 View
- 24 Download
-
Abstract
PDF
Supplementary Material -
The pho regulon plays a critical role in maintaining phosphate homeostasis in bacteria, with the PhoU protein functioning as a regulator that bridges the PhoB/PhoR two-component system and the PstSCAB2 phosphate transporter. While PhoU is known to suppress PhoR autophosphorylation under high phosphate conditions via interaction with its PAS domain, its broader regulatory functions remain elusive. Here, we investigated the role of the PhoU Ala147 residue in Salmonella enterica serovar Typhimurium using a phoUA147E substitution mutant. Bacterial two-hybrid and immunoprecipitation assays confirmed that Ala147 is essential for PhoU-PhoR PAS domain interaction, and its substitution leads to derepression of pho regulon genes, even in high phosphate conditions. This disruption impaired Salmonella survival inside macrophages and mouse virulence, demonstrating the importance of PhoU-PhoR interaction in Salmonella pathogenesis. However, unlike the phoU deletion mutant, the phoUA147E mutant does not exhibit growth defects or polyphosphate accumulation, indicating that the PhoU-PhoR interaction is not involved in these phenotypes. Our findings reveal PhoU as a multifaceted regulator, coordinating phosphate uptake and pho regulon expression through distinct molecular interactions, and provide new insights into its role in bacterial physiology and virulence.
Research Support, Non-U.S. Gov't
- Role of a Burkholderia pseudomallei Polyphosphate Kinase in an Oxidative Stress Response, Motilities, and Biofilm Formation
- Suda Tunpiboonsak , Rungrawee Mongkolrob , Kaniskul Kitudomsub , Phawatwaristh Thanwatanaying , Witcha Kiettipirodom , Yanin Tungboontina , Sumalee Tungpradabkul
- J. Microbiol. 2010;48(1):63-70. Published online March 11, 2010
- DOI: https://doi.org/10.1007/s12275-010-9138-5
- 199 View
- 0 Download
- 40 Crossref
-
Abstract
PDF
- Burkholderia pseudomallei, a motile and rod Gram-negative bacterium, is the causative agent of melioidosis. The bacterium is an intracellular pathogen and that motility is generally crucial for their survival in a natural environment and for systemic infection inside a host. We report here a role of B. pseudomallei polyphosphate kinase in virulence, such as an oxidative stress response, motilities and biofilm formation. The polyphosphate kinase (ppk) mutant is susceptible to hydrogen peroxide in an oxidative stress condition, unable to perform swimming, swarming motilities, and has lower density biofilm forming capacity than the wild-type strain. We also demonstrated that both polyphosphate kinase and motile flagella are essential and independently involved in biofilm formation. The B. pseudomallei flagellin (fliC) mutant and B. mallei, a nonmotile species, are shown to produce higher density biofilm formation than the ppk mutant, but less than wild type B. pseudomallei.
-
Citations
Citations to this article as recorded by
- Inorganic Polyphosphate Relieves Ulcerative Colitis by Modulating the Gut Microbiota and Metabolites
Zhicheng Wang, Jing Zhao, Sisi He, Rongpeng Li, Xiuxiu Wang, Chao Yan, Jing Zhao, Wei Wei
ACS Applied Bio Materials.2025; 8(8): 7473. CrossRef - C-terminal Poly-histidine Tags Alter Escherichia coli Polyphosphate Kinase Activity and Susceptibility to Inhibition
Marvin Q. Bowlin, Avery D. Lieber, Abagail R. Long, Michael J. Gray
Journal of Molecular Biology.2024; 436(16): 168651. CrossRef - Gallein potentiates isoniazid's ability to suppress Mycobacterium tuberculosis growth
Ramesh Rijal, Richard H. Gomer
Frontiers in Microbiology.2024;[Epub] CrossRef - Transcriptional landscape of Burkholderia pseudomallei cultured under environmental and clinical conditions
Cin Kong, Rui-Rui Wong, Ahmad-Kamal Ghazali, Yuka Hara, Tengku Nurfarhana Tengku Aziz, Sheila Nathan
Microbial Genomics .2023;[Epub] CrossRef - Upgrade the high-load anaerobic digestion and relieve acid stress through the strategy of side-stream micro-aeration: biochemical performances, microbial response and intrinsic mechanisms
Wen Li, Yongli Liu, Baocun Wu, Li Gu, Rui Deng
Water Research.2022; 221: 118850. CrossRef - Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples
Siti Nur Hazwani Oslan, Abdul Hafidz Yusoff, Mazlina Mazlan, Si Jie Lim, Jing Jing Khoo, Siti Nurbaya Oslan, Aziah Ismail
Microbial Pathogenesis.2022; 169: 105637. CrossRef - The GntR-like transcriptional regulator HutC involved in motility, biofilm-forming ability, and virulence in Vibrio parahaemolyticus
Yangyang Li, Weidong Sun, Quan Wang, Ying Yu, Ying Wan, Kai Zhou, Rong Guo, Xiangan Han, Zhaoguo Chen, Weihuan Fang, Wei Jiang
Microbial Pathogenesis.2022; 167: 105546. CrossRef - Inorganic polyphosphate in host and microbe biology
Marvin Q. Bowlin, Michael J. Gray
Trends in Microbiology.2021; 29(11): 1013. CrossRef - Proteomics insights into the Burkholderia cenocepacia phosphorus stress response
Holly Shropshire, Rebekah A. Jones, María M. Aguilo‐Ferretjans, David J. Scanlan, Yin Chen
Environmental Microbiology.2021; 23(9): 5069. CrossRef - In vitro passage alters virulence, immune activation and proteomic profiles of Burkholderia pseudomallei
Taksaon Duangurai, Onrapak Reamtong, Amporn Rungruengkitkun, Varintip Srinon, Usa Boonyuen, Direk Limmathurotsakul, Narisara Chantratita, Pornpan Pumirat
Scientific Reports.2020;[Epub] CrossRef - Polyphosphate is an extracellular signal that can facilitate bacterial survival in eukaryotic cells
Ramesh Rijal, Louis A. Cadena, Morgan R. Smith, Joseph F. Carr, Richard H. Gomer
Proceedings of the National Academy of Sciences.2020; 117(50): 31923. CrossRef -
Peptidyl-Prolyl Isomerase
ppiB
Is Essential for Proteome Homeostasis and Virulence in Burkholderia pseudomallei
Nicole M. Bzdyl, Nichollas E. Scott, Isobel H. Norville, Andrew E. Scott, Timothy Atkins, Stanley Pang, Derek S. Sarovich, Geoffrey Coombs, Timothy J. J. Inglis, Charlene M. Kahler, Mitali Sarkar-Tyson, Manuela Raffatellu
Infection and Immunity.2019;[Epub] CrossRef - Polyphosphate kinase 1 of Burkholderia pseudomallei controls quorum sensing, RpoS and host cell invasion
Kitima Srisanga, Praewa Suthapot, Permkun Permsirivisarn, Piyarat Govitrapong, Sumalee Tungpradabkul, Patompon Wongtrakoongate
Journal of Proteomics.2019; 194: 14. CrossRef - Bacterial Polyphosphate Kinases Revisited: Role in Pathogenesis and Therapeutic Potential
Lalit Kumar Gautam, Prince Sharma, Neena Capalash
Current Drug Targets.2019; 20(3): 292. CrossRef - BIOFILMS OF PATHOGENIC BURKHOLDERIA AND THEIR ROLE IN RESISTANCE TO ANTIBIOTICS
E. V. Shubnikova, L. K. Merinova, T. V. Senina, E. V. Korol, O. A. Merinova
Journal of microbiology, epidemiology and immunobiology.2018; 95(1): 101. CrossRef - Genome-scale analysis of the genes that contribute to Burkholderia pseudomallei biofilm formation identifies a crucial exopolysaccharide biosynthesis gene cluster
Grace I. Borlee, Brooke A. Plumley, Kevin H. Martin, Nawarat Somprasong, Mihnea R. Mangalea, M. Nurul Islam, Mary N. Burtnick, Paul J. Brett, Ivo Steinmetz, David P. AuCoin, John T. Belisle, Dean C. Crick, Herbert P. Schweizer, Bradley R. Borlee, Nicholas
PLOS Neglected Tropical Diseases.2017; 11(6): e0005689. CrossRef - Paraburkholderia phytofirmans PsJN Protects Arabidopsis thaliana Against a Virulent Strain of Pseudomonas syringae Through the Activation of Induced Resistance
Tania Timmermann, Grace Armijo, Raúl Donoso, Aldo Seguel, Loreto Holuigue, Bernardo González
Molecular Plant-Microbe Interactions®.2017; 30(3): 215. CrossRef - Complete genome analysis of Lactobacillus fermentum SK152 from kimchi reveals genes associated with its antimicrobial activity
DongAhn Yoo, Bernadette B. Bagon, Valerie Diane V. Valeriano, Ju Kyoung Oh, Heebal Kim, Seoae Cho, Dae-Kyung Kang
FEMS Microbiology Letters.2017;[Epub] CrossRef - Ultrastructural effects and antibiofilm activity of LFchimera against Burkholderia pseudomallei
Aekkalak Puknun, Sakawrat Kanthawong, Chitchanok Anutrakunchai, Kamran Nazmi, Wikky Tigchelaar, Kees A. Hoeben, Enno C. I. Veerman, Jan G. M. Bolscher, Suwimol Taweechaisupapong
World Journal of Microbiology and Biotechnology.2016;[Epub] CrossRef - Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033
Jing Chen, Lijie Su, Xiangru Wang, Tao Zhang, Feng Liu, Huanchun Chen, Chen Tan
Frontiers in Microbiology.2016;[Epub] CrossRef - A predicted cation transporter protein, BPSS1228, is involved in intracellular behaviour ofBurkholderia pseudomalleiin a human lung epithelial cell line (A549)
Teerasit Techawiwattanaboon, Sorujsiri Chareonsudjai, Craig Winstanley
FEMS Microbiology Letters.2016; 363(23): fnw259. CrossRef - Biochemical and structural characterization of polyphosphate kinase 2 from the intracellular pathogen Francisella tularensis
Laura E. Batten, Alice E. Parnell, Neil J. Wells, Amber L. Murch, Petra C. F. Oyston, Peter L. Roach
Bioscience Reports.2016;[Epub] CrossRef - Two-Phase Bactericidal Mechanism of Silver Nanoparticles against Burkholderia pseudomallei
Pawinee Siritongsuk, Nuttaya Hongsing, Saengrawee Thammawithan, Sakda Daduang, Sompong Klaynongsruang, Apichai Tuanyok, Rina Patramanon, Lisa A. Morici
PLOS ONE.2016; 11(12): e0168098. CrossRef - Perturbation of the two-component signal transduction system, BprRS, results in attenuated virulence and motility defects in Burkholderia pseudomallei
Natalie R. Lazar Adler, Elizabeth M. Allwood, Deanna Deveson Lucas, Paul Harrison, Stephen Watts, Alexandra Dimitropoulos, Puthayalai Treerat, Priyangi Alwis, Rodney J. Devenish, Mark Prescott, Brenda Govan, Ben Adler, Marina Harper, John D. Boyce
BMC Genomics.2016;[Epub] CrossRef - Ellagic acid derivatives from Terminalia chebula
Retz. increase the susceptibility of Pseudomonas aeruginosa
to stress by inhibiting polyphosphate kinase
S. Sarabhai, K. Harjai, P. Sharma, N. Capalash
Journal of Applied Microbiology.2015; 118(4): 817. CrossRef - Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress
Ricardo Jasso-Chávez, M. Geovanni Santiago-Martínez, Elizabeth Lira-Silva, Erika Pineda, Armando Zepeda-Rodríguez, Javier Belmont-Díaz, Rusely Encalada, Emma Saavedra, Rafael Moreno-Sánchez, Stephan Neil Witt
PLOS ONE.2015; 10(2): e0117331. CrossRef - Global transcriptional analysis of Burkholderia pseudomallei high and low biofilm producers reveals insights into biofilm production and virulence
Chui-Yoke Chin, Yuka Hara, Ahmad-Kamal Ghazali, Soon-Joo Yap, Cin Kong, Yee-Chin Wong, Naufal Rozali, Seng-Fook Koh, Chee-Choong Hoh, Savithri D. Puthucheary, Sheila Nathan
BMC Genomics.2015;[Epub] CrossRef - Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomalleibpsI, ppk, and rpoS mutant strains
Rungrawee Mongkolrob, Suwimol Taweechaisupapong, Sumalee Tungpradabkul
Microbiology and Immunology.2015; 59(11): 653. CrossRef - Utilization of Whole-Cell MALDI-TOF Mass Spectrometry to Differentiate Burkholderia pseudomallei Wild-Type and Constructed Mutants
Suthamat Niyompanich, Kitima Srisanga, Janthima Jaresitthikunchai, Sittiruk Roytrakul, Sumalee Tungpradabkul, Yang Cai
PLOS ONE.2015; 10(12): e0144128. CrossRef - What Drives the Occurrence of the Melioidosis Bacterium Burkholderia pseudomallei in Domestic Gardens?
Mirjam Kaestli, Glenda Harrington, Mark Mayo, Mark D. Chatfield, Ian Harrington, Audrey Hill, Niels Munksgaard, Karen Gibb, Bart J. Currie, Joseph M. Vinetz
PLOS Neglected Tropical Diseases.2015; 9(3): e0003635. CrossRef - Deficiency of the Novel Exopolyphosphatase Rv1026/PPX2 Leads to Metabolic Downshift and Altered Cell Wall Permeability in Mycobacterium tuberculosis
Yu-Min Chuang, Nirmalya Bandyopadhyay, Dalin Rifat, Harvey Rubin, Joel S. Bader, Petros C. Karakousis, Christina Stallings, L. David Sibley
mBio.2015;[Epub] CrossRef - Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner
Carmen C. Denman, Matthew T. Robinson, Andrea M. Sass, Eshwar Mahenthiralingam, Alan R. Brown
Microbiology.2014; 160(1): 187. CrossRef - The Multiple Roles of Hypothetical Gene BPSS1356 in Burkholderia pseudomallei
Hokchai Yam, Ainihayati Abdul Rahim, Suriani Mohamad, Nor Muhammad Mahadi, Uyub Abdul Manaf, Alexander Chong Shu-Chien, Nazalan Najimudin, Catherine A. Brissette
PLoS ONE.2014; 9(6): e99218. CrossRef - Functional characterization of exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) ofCampylobacter jejuni
Anandkumar Malde, Dharanesh Gangaiah, Kshipra Chandrashekhar, Ruby Pina-Mimbela, Jordi B Torrelles, Gireesh Rajashekara
Virulence.2014; 5(4): 521. CrossRef - Identification of a Predicted Trimeric Autotransporter Adhesin Required for Biofilm Formation of Burkholderia pseudomallei
Natalie R. Lazar Adler, Rachel E. Dean, Richard J. Saint, Mark P. Stevens, Joann L. Prior, Timothy P. Atkins, Edouard E. Galyov, Lisa A. Morici
PLoS ONE.2013; 8(11): e79461. CrossRef - A heterodimer comprised of two bovine lactoferrin antimicrobial peptides exhibits powerful bactericidal activity against Burkholderia pseudomallei
Aekkalak Puknun, Jan G. M. Bolscher, Kamran Nazmi, Enno C. I. Veerman, Sumalee Tungpradabkul, Surasakdi Wongratanacheewin, Sakawrat Kanthawong, Suwimol Taweechaisupapong
World Journal of Microbiology and Biotechnology.2013; 29(7): 1217. CrossRef - Bacterial Phosphate Homeostasis: Role of Phosphate Transporters
Yoon-Mee Park, Iel-Soo Bang
The Korean Journal of Microbiology.2012; 48(2): 57. CrossRef - Regulation of a quorum sensing system by stationary phase sigma factor RpoS and their co‐regulation of target genes
in Burkholderia pseudomallei
Patompon Wongtrakoongate, Sarinna Tumapa, Sumalee Tungpradabkul
Microbiology and Immunology.2012; 56(5): 281. CrossRef - Polyphosphate Deficiency Affects the Sliding Motility and Biofilm Formation of Mycobacterium smegmatis
Tingyu Shi, Tiwei Fu, Jianping Xie
Current Microbiology.2011; 63(5): 470. CrossRef - Biofilm formation in bacterial pathogens of veterinary importance
Mario Jacques, Virginia Aragon, Yannick D. N. Tremblay
Animal Health Research Reviews.2010; 11(2): 97. CrossRef
- Inorganic Polyphosphate Relieves Ulcerative Colitis by Modulating the Gut Microbiota and Metabolites
First
Prev
TOP
