Journal of Microbiology

Characterization of the Proteolytic Activity of Bacteria Isolated from a Rotating Biological Contactor: In Jae Park , Jerng Chang Yoon , Seong Joo Park , Eung Ho Kim , Yeon-Jae Cho , Kwang-Soo Shin; J. Microbiol. 2003;41(2):73-77.

Abstract: Four proteolytic bacteria were isolated and identified from a rotating biological contactor based on Bacillus. The four isolates, Ni 26, 36, 39 and 49 were identified as B. vallismortis, B. subtilis, Aeromonas hydrophila and B. amyloliquefaciens, respectively, based on their biochemical properties and 16S rDNA sequence analyses. The optimal proteolytic activity was observed in the temperature and pH ranges of 40-70ºC and 8.0-8.5, respectively. The proteolytic activities of all the isolates were partially inhibited by phenylmethylsulfonylfluoride (PMSF), and the isolates Ni 26, Ni 39 and Ni 49 were inhibited by the metalloprotease inhibitor, 1,10-phenanthroline. Zymographic analyses of the culture supernatants revealed the presence of at least two proteases in all isolates.

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