Journal of Microbiology

Research Support, Non-U.S. Gov't

Potentiation of Bacterial Killing Activity of Zinc Chloride by Pyrrolidine Dithiocarbamate: Eun-Kyoung Choi , Hye-Hyang Lee , Mi-Sun Kang , Byung-Gook Kim , Hoi-Soon Lim , Seon-Mi Kim , In-Chol Kang; J. Microbiol. 2010;48(1):40-43. Published online March 11, 2010; DOI: https://doi.org/10.1007/s12275-009-0049-2

Abstract: Zinc has antimicrobial activity and zinc salts including zinc chloride (ZnCl2) have been used for the control of oral malodor. In this study, we hypothesized that pyrrolidine dithiocarbamate (PDTC), a zinc ionophore, may enhance antimicrobial efficacy of ZnCl2. The bactericidal effectiveness of ZnCl2 alone (0.5-8 mM) or in combination with PDTC (1 or 10 μM) was evaluated by in vitro short (1 h) time-killing assays against Fusobacterium nucleatum and Porphyromonas gingivalis. Only a slight viability decrease was observed with ZnCl2 or PDTC alone after 1-h incubation. By contrast, combination of ZnCl2 and PDTC could achieve a more than 100-fold viability reduction compared with ZnCl2 or PDTC alone in F. nucleatum and P. gingivalis. Therefore, PDTC greatly enhanced the bactericidal activity of ZnCl2 against the oral malodor-producing bacteria. These results suggest that use of PDTC may be useful for enhancing bactericidal activity of antimalodor regimens of zinc salts.

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