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- σB Affects Biofilm Formation under the Dual Stress Conditions Imposed by Adding Salt and Low Temperatur in Listeria monocytogenes
- Jin-Ju Lee , Gilho Lee , Ji-Hyun Shin
- J. Microbiol. 2014;52(10):849-855. Published online October 1, 2014
- DOI: https://doi.org/10.1007/s12275-014-4369-5
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- 10 Crossref
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Abstract
- The food-borne pathogenic bacteria Listeria monocytogenes can form biofilms on various surfaces including food-processing equipment. Biofilms offer survival benefits to the organisms entrapped against environmental insults. Moreover, the σB transcription factor of L. monocytogenes plays an important role in its survival under various stress conditions. In this study, we evaluated whether σB contributes to biofilm formation when L. monocytogenes is grown under various temperatures and media. When the wild-type strain was grown under static biofilm culture below ambient temperature (15°C) for 72 h, the difference in viable cell number (in both planktonic and biofilm cells) between the wild-type and ΔsigB mutant increased by adding NaCl to BHI broth (9% salt BHI > 6% salt BHI > BHI, w/v), and the specific activity of β-galactosidase was highly induced in the wild-type strain grown in 6% salt containing BHI broth. Furthermore, we measured surface-adhered biofilm forming ability using the crystal violet staining method. The wild-type strain formed a four times larger biofilm than that of the ΔsigB mutant in 6% salt-BHI medium at 15°C over a 72 h incubation and also showed the highest level of β-galactosidase specific activity. However, both the wild-type and ΔsigB mutant L. monocytogenes were defective for forming a biofilm in 9% salt-BHI medium at 15°C. Our results suggest that σB plays an enhanced role in surface-adhered biofilm formation when L. monocytogenes encounters dual stress conditions, such as 6% NaCl and low temperature.
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Citations
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- Characteristics of Clinical Isolates of Listeria monocytogenes in Sichuan, China, in 2022 Based on Whole Genome Sequencing Analysis
Qian Liang, Weifeng Huang, Tao Xiao, Lin Zhang, Gaopeng Lei, Hong Lv, Xiaorong Yang
Foodborne Pathogens and Disease.2024; 21(7): 424. CrossRef - The influence of stress factors on selected phenotypic and genotypic features of Listeria monocytogenes – a pilot study
Natalia Wiktorczyk-Kapischke, Ewa Wałecka-Zacharska, Jakub Korkus, Katarzyna Grudlewska-Buda, Anna Budzyńska, Kacper Wnuk, Eugenia Gospodarek-Komkowska, Krzysztof Skowron
BMC Microbiology.2023;[Epub] CrossRef - Marked inter-strain heterogeneity in the differential expression of some key stress response and virulence-related genes between planktonic and biofilm cells in Listeria monocytogenes
Christos Toliopoulos, Efstathios Giaouris
International Journal of Food Microbiology.2023; 390: 110136. CrossRef - Proteomic analysis reveals the temperature-dependent presence of extracytoplasmic peptidases in the biofilm exoproteome of Listeria monocytogenes EGD-e
Yue-Jia Lee, Chinling Wang
Journal of Microbiology.2020; 58(9): 761. CrossRef - Genomic Differences between Listeria monocytogenes EGDe Isolates Reveal Crucial Roles for SigB and Wall Rhamnosylation in Biofilm Formation
Chih-Yu Hsu, Lynne Cairns, Laura Hobley, James Abbott, Conor O’Byrne, Nicola R. Stanley-Wall, Tina M. Henkin
Journal of Bacteriology.2020;[Epub] CrossRef - Listeria monocytogenes Biofilm Adaptation to Different Temperatures Seen Through Shotgun Proteomics
Tiago Santos, Didier Viala, Christophe Chambon, Julia Esbelin, Michel Hébraud
Frontiers in Nutrition.2019;[Epub] CrossRef - Cross Talk between SigB and PrfA in Listeria monocytogenes Facilitates Transitions between Extra- and Intracellular Environments
Ahmed Gaballa, Veronica Guariglia-Oropeza, Martin Wiedmann, Kathryn J. Boor
Microbiology and Molecular Biology Reviews.2019;[Epub] CrossRef - Salt stress affects global protein expression profiles of extracellular membrane-derived vesicles of Listeria monocytogenes
Taewon Lee, So Hyun Jun, Chi Won Choi, Seung Il Kim, Je Chul Lee, Ji Hyun Shin
Microbial Pathogenesis.2018; 115: 272. CrossRef - Genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (VpaCspA and VpaCspD) in Vibrio parahaemolyticus CHN25 during low-temperature survival
Chunhua Zhu, Boyi Sun, Taigang Liu, Huajun Zheng, Wenyi Gu, Wei He, Fengjiao Sun, Yaping Wang, Meicheng Yang, Weicheng Bei, Xu Peng, Qunxin She, Lu Xie, Lanming Chen
BMC Genomics.2017;[Epub] CrossRef - Regulation of Escherichia coli RNase III activity
Boram Lim, Minji Sim, Howoon Lee, Seogang Hyun, Younghoon Lee, Yoonsoo Hahn, Eunkyoung Shin, Kangseok Lee
Journal of Microbiology.2015; 53(8): 487. CrossRef
- Characteristics of Clinical Isolates of Listeria monocytogenes in Sichuan, China, in 2022 Based on Whole Genome Sequencing Analysis
- Expression Analysis of LeNHX1 Gene in Mycorrhizal Tomato under Salt Stress
- ZhongQun He , Zhi Huang
- J. Microbiol. 2013;51(1):100-104. Published online March 2, 2013
- DOI: https://doi.org/10.1007/s12275-013-2423-3
- 37 View
- 0 Download
- 17 Scopus
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Abstract
- The plant growth, stem sap flow, Na+ and Cl- content, and the expression of vacuolar Na+/H+ antiporter gene (LeNHX1) in the leaves and roots of tomato under different NaCl stresses (0.5% and 1%) were studied to analyze the effect of arbuscular mycorrhizal fungi (AMF) on Na+ and Cl- accumulation and ion exchange. The results showed that arbuscular mycorrhizal (AM) plant growth and stem sap flow increased and salt tolerance improved, whereas Na+ and Claccumulated. Na+ significantly decreased, and no significant decline was detected in Cl- content after AMF inoculation compared with the non-AM plants. The LeNHX1 gene expression was induced in the AM and non-AM plants by NaCl stress. However, AMF did not improve the LeNHX1 level, and low expression was observed in the AM tomato. Hence, the mechanism that reduced the Na+ damage to tomato induced by AMF has little relation to LeNHX1, which can export Na+ from the cytosol to the vacuole across the tonoplast.
- Evidence That Vibrio vulnificus AhpC2 Is Essential for Survival Under High Salinity by Modulating Intracellular Level of ROS
- Myung Jin Koh , Hyun Sung Lee , Jee Eun Rhee , Sang Ho Choi
- J. Microbiol. 2010;48(1):129-133. Published online March 11, 2010
- DOI: https://doi.org/10.1007/s12275-009-0227-2
- 26 View
- 0 Download
- 5 Scopus
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Abstract
- Expression of ahpC2 encoding an alkyl hydroperoxide reductase of Vibrio vulnificus, a foodborne pathogen, was incrementally induced depending on NaCl concentrations in the culture. Growth of the ahpC2 mutant was significantly impaired with longer lag phase and lower growth rate when cultured under high salinity. ROS was accumulated in V. vulnificus cells when stressed by exposure to high salinity, and the ahpC2 mutant accumulated higher level of ROS as compared with the parental wild type. Consequently, the combined results suggest that AhpC2 contributes to the growth of V. vulnificus under high salinity by scavenging ROS in cells.
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