Effect of Iron on Cytolysin A Expression in Salmonella enterica serovar Typhi

Jinghua Cui; Honghua Piao; Shen Jin; Hee Sam Na; Yeongjin Hong; Hyon E Choy; Phil Youl Ryu

doi:10.1007/s12275-009-0039-4

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Research Support, Non-U.S. Gov't
Effect of Iron on Cytolysin A Expression in Salmonella enterica serovar Typhi: Jinghua Cui ^1,2,3,4, Honghua Piao ^1,3, Shen Jin ^1,2,3,4, Hee Sam Na ¹, Yeongjin Hong ^1,2,3, Hyon E Choy ^1,2,3, Phil Youl Ryu ^1,2,3; Journal of Microbiology 2009;47(4):479-485.
DOI: https://doi.org/10.1007/s12275-009-0039-4
Published online: September 9, 2009

¹Department of Microbiology, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, ²Research Center for Controlling Microbial Virulence, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, ³Clinical Vaccine R&D Center, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea, ⁴the Brain Korea 21 Project, Center for Biomedical Human Resources, Chonnam National University Medical School, Gwangju 501-746, Republic of Korea

Corresponding author: Phil Youl Ryu , Tel: 82-62-220-4135,

Received: 9 February 2009 • Accepted: 29 March 2009

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Abstract

Previously, a novel protein ClyA (Cytolysin A) has been identified in Escherichia coli K-12, Salmonella enterica serovars Typhi and Paratyphi A and Shigella. Salmonella spp. synthesize substantial amounts of ClyA upon infection of the human host, although the mechanism by which ClyA is induced in vivo is unclear. Since environmental signals could control the expression of virulence determinants, ClyA expression in S. Typhi Ty2 was tested by Western blotting in the presence of normal pooled human serum (NPS). The level of ClyA expression increased in the presence of NPS in a concentration-dependent manner. RPMI 1640 medium similarly induced ClyA expression. ClyA expression was inversely proportional to the concentration of iron in RPMI medium. Therefore, we speculated that iron inhibited the expression of ClyA in S. Typhi Ty2, and free iron depletion may be one of the causes of S. Typhi-mediated induction of ClyA in vivo. Transcription from a clyA-lacZ fusion gene decreased as iron concentration increased, but not as significantly as the ClyA protein expression. It is concluded that the regulatory effect of iron on clyA expression is mainly at translational level.

Keywords: cytolysin A (ClyA);iron;S. Typhi Ty2

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Expression of Bacteroides fragilis hemolysins in vivo and role of HlyBA in an intra‐abdominal infection model
Leandro A. Lobo, Audrey L. Jenkins, C. Jeffrey Smith, Edson R. Rocha
MicrobiologyOpen.2013; 2(2): 326. CrossRef
Molecular cloning and characterization of clyA genes in various serotypes of Salmonella enterica
Lan Ji Huang, Jinghua Cui, Hong Hua Piao, Yeongjin Hong, Hyon E. Choy, Phil Youl Ryu
The Journal of Microbiology.2010; 48(5): 663. CrossRef

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Effect of Iron on Cytolysin A Expression in Salmonella enterica serovar Typhi

J. Microbiol. 2009;47(4):479-485. Published online September 9, 2009

DOI: https://doi.org/10.1007/s12275-009-0039-4

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