Transmission of Methylobacterium mesophilicum by Bucephalogonia xanthophis for Paratransgenic Control Strategy of Citrus Variegated Chlorosis

Cláudia Santos Gai; Paulo Teixeira Lacava; Maria Carolina Quecine; Marie-Christine Auriac; João Roberto Spotti Lopes; Welington Luiz Araújo; Thomas Albert Miller; João Lúcio Azevedo

doi:10.1007/s12275-008-0303-z

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Transmission of Methylobacterium mesophilicum by Bucephalogonia xanthophis for Paratransgenic Control Strategy of Citrus Variegated Chlorosis: Cláudia Santos Gai ¹, Paulo Teixeira Lacava ¹, Maria Carolina Quecine ¹, Marie-Christine Auriac ², João Roberto Spotti Lopes ³, Welington Luiz Araújo ¹, Thomas Albert Miller ⁴, João Lúcio Azevedo ¹; Journal of Microbiology 2009;47(4):448-454.
DOI: https://doi.org/10.1007/s12275-008-0303-z
Published online: September 9, 2009

¹Department of Genetics, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo, 13400-970 Piracicaba, SP, Brazil, ²Laboratoire des Interaction Plantes Microorganismes, INRA/CNRS, 31326 Castanet-Tolosan, France, ³Department of Entomology, Plant Pathology and Agricultural Zoology, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo, 13418-900 Piracicaba, SP, Brazil, ⁴Department of Entomology, University of California Riverside, 9252 Riverside, CA, USA¹Department of Genetics, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo, 13400-970 Piracicaba, SP, Brazil, ²Laboratoire des Interaction Plantes Microorganismes, INRA/CNRS, 31326 Castanet-Tolosan, France, ³Department of Entomology, Plant Pathology and Agricultural Zoology, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo, 13418-900 Piracicaba, SP, Brazil, ⁴Department of Entomology, University of California Riverside, 9252 Riverside, CA, USA

Corresponding author: Paulo Teixeira Lacava , Tel: 55-19-3429-4251,

Received: 16 December 2008 • Accepted: 31 March 2009

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Abstract

Methylobacterium mesophilicum, originally isolated as an endophytic bacterium from citrus plants, was genetically transformed to express green fluorescent protein (GFP). The GFP-labeled strain of M. mesophilicum was inoculated into Catharanthus roseus (model plant) seedlings and further observed colonizing its xylem vessels. The transmission of this endophyte by Bucephalogonia xanthophis, one of the insect vectors that transmit Xylella fastidiosa subsp. pauca, was verified by insects feeding from fluids containing the GFP bacterium followed by transmission to plants and isolating the endophyte from C. roseus plants. Forty-five days after inoculation, the plants exhibited endophytic colonization by M. mesophilicum, confirming this bacterium as a nonpathogenic, xylem-associated endophyte. Our data demonstrate that M. mesophilicum not only occupy the same niche of X. fastidiosa subsp. pauca inside plants but also may be transmitted by B. xanthophis. The transmission, colonization, and genetic manipulation of M. mesophilicum is a prerequisite to examining the potential use of symbiotic control to interrupt the transmission of X. fastidiosa subsp. pauca, the bacterial pathogen causing Citrus variegated chlorosis by insect vectors.

Keywords: endophytic symbionts;Citrus sinensis;Methylobacterium mesophilicum;sharpshooters;symbiotic control;Xylella fastidiosa subsp. pauca

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Transmission of Methylobacterium mesophilicum by Bucephalogonia xanthophis for Paratransgenic Control Strategy of Citrus Variegated Chlorosis

J. Microbiol. 2009;47(4):448-454. Published online September 9, 2009

DOI: https://doi.org/10.1007/s12275-008-0303-z

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