NOTE] Evidence Against the Physiological Role of Acetyl Phosphate in the Phosphorylation of the ArcA Response Regulator in Escherichia coli

Xueqiao Liu; Gabriela R. Peña Sandoval; Barry L. Wanner; Won Seok Jung; Dimitris Georgellis; Ohsuk Kwon

doi:10.1007/s12275-009-0087-9

Articles

Page Path: HOME > J. Microbiol > Volume 47(5); 2009 > Article

Research Support, Non-U.S. Gov't
NOTE] Evidence Against the Physiological Role of Acetyl Phosphate in the Phosphorylation of the ArcA Response Regulator in Escherichia coli: Xueqiao Liu ¹, Gabriela R. Peña Sandoval ², Barry L. Wanner ³, Won Seok Jung ⁴, Dimitris Georgellis ², Ohsuk Kwon ⁴; Journal of Microbiology 2009;47(5):657-662
DOI: https://doi.org/10.1007/s12275-009-0087-9
Published online: October 24, 2009

¹Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA, ²Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico, ³Department of Biological Sciences, Purdue University, Lafayette, IN 47907, USA, ⁴Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea¹Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA, ²Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico, ³Department of Biological Sciences, Purdue University, Lafayette, IN 47907, USA, ⁴Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea

Corresponding author: Ohsuk Kwon , Tel: 82-42-860-4459,

Received: 20 March 2009 • Accepted: 24 June 2009

6 Views
0 Download
0 Crossref
16 Scopus

Full Article

Abstract

The Arc two-component signal transduction system of Escherichia coli comprises the ArcB sensor kinase and the ArcA response regulator. Under anoxic growth conditions, ArcB autophosphorylates and transphosphorylates ArcA, which, in turn, represses or activates its target operons. ArcA has been shown to be able to autophosphorylate in vitro at the expense of acetyl-P. Here, the in vivo effect of acetyl phosphate on the redox signal transduction by the Arc system was assessed. Our results indicate that acetyl phosphate can modulate the expression of ArcA-P target genes only in the absence of ArcB. Therefore, the acetyl phosphate dependent ArcA phosphorylation route does not seem to play a significant role under physiological conditions.

Keywords: acetyl phosphate; Arc two-component signal transduction; ArcA response regulator; E. coli

Figure & Data

References

Citations

Citations to this article as recorded by

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

NOTE] Evidence Against the Physiological Role of Acetyl Phosphate in the Phosphorylation of the ArcA Response Regulator in Escherichia coli

J. Microbiol. 2009;47(5):657-662. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-009-0087-9

Related articles