NOTE] Evidence Against the Physiological Role of Acetyl Phosphate in the Phosphorylation of the ArcA Response Regulator in Escherichia coli

Xueqiao Liu; Gabriela R. Peña Sandoval; Barry L. Wanner; Won Seok Jung; Dimitris Georgellis; Ohsuk Kwon

doi:10.1007/s12275-009-0087-9

Articles

Page Path: HOME > J. Microbiol > Volume 47(5); 2009 > Article

Research Support, Non-U.S. Gov't
NOTE] Evidence Against the Physiological Role of Acetyl Phosphate in the Phosphorylation of the ArcA Response Regulator in Escherichia coli: Xueqiao Liu ¹, Gabriela R. Peña Sandoval ², Barry L. Wanner ³, Won Seok Jung ⁴, Dimitris Georgellis ², Ohsuk Kwon ⁴; Journal of Microbiology 2009;47(5):657-662.
DOI: https://doi.org/10.1007/s12275-009-0087-9
Published online: October 24, 2009

¹Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA, ²Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico, ³Department of Biological Sciences, Purdue University, Lafayette, IN 47907, USA, ⁴Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea¹Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA, ²Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico, ³Department of Biological Sciences, Purdue University, Lafayette, IN 47907, USA, ⁴Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea

Corresponding author: Ohsuk Kwon , Tel: 82-42-860-4459,

Received: 20 March 2009 • Accepted: 24 June 2009

37 Views
0 Download
12 Crossref
16 Scopus

Full Article

Abstract

The Arc two-component signal transduction system of Escherichia coli comprises the ArcB sensor kinase and the ArcA response regulator. Under anoxic growth conditions, ArcB autophosphorylates and transphosphorylates ArcA, which, in turn, represses or activates its target operons. ArcA has been shown to be able to autophosphorylate in vitro at the expense of acetyl-P. Here, the in vivo effect of acetyl phosphate on the redox signal transduction by the Arc system was assessed. Our results indicate that acetyl phosphate can modulate the expression of ArcA-P target genes only in the absence of ArcB. Therefore, the acetyl phosphate dependent ArcA phosphorylation route does not seem to play a significant role under physiological conditions.

Keywords: acetyl phosphate;Arc two-component signal transduction;ArcA response regulator;E. coli

Figure & Data

References

Citations

Citations to this article as recorded by

Theoretical study of ArcB and its dimerization, interaction with anaerobic metabolites, and activation of ArcA
Felipe Padilla-Vaca, Javier de la Mora, Rodolfo García-Contreras, Jorge Humberto Ramírez-Prado, Marcos Vicente-Gómez, Francisco Vargas-Gasca, Fernando Anaya-Velázquez, Itzel Páramo-Pérez, Ángeles Rangel-Serrano, Patricia Cuéllar-Mata, Naurú Idalia Vargas-
PeerJ.2023; 11: e16309. CrossRef
The role of sensory kinase proteins in two-component signal transduction
Adrián F. Alvarez, Dimitris Georgellis
Biochemical Society Transactions.2022; 50(6): 1859. CrossRef
The ArcAB Two-Component System: Function in Metabolism, Redox Control, and Infection
Aric N. Brown, Mark T. Anderson, Michael A. Bachman, Harry L. T. Mobley
Microbiology and Molecular Biology Reviews.2022;[Epub] CrossRef
Acetate and Potassium Modulate the Stationary-Phase Activation of lrgAB in Streptococcus mutans
Sang-Joon Ahn, Shailja Desai, Loraine Blanco, Min Lin, Kelly C. Rice
Frontiers in Microbiology.2020;[Epub] CrossRef
Identification of Z nucleotides as an ancient signal for two-component system activation in bacteria
Oscar J. Vázquez-Ciros, Adrián F. Alvarez, Dimitris Georgellis
Proceedings of the National Academy of Sciences.2020; 117(52): 33530. CrossRef
Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways
Atsumi Hirose, Takuya Kasai, Motohide Aoki, Tomonari Umemura, Kazuya Watanabe, Atsushi Kouzuma
Nature Communications.2018;[Epub] CrossRef
Organization and mode of action of two component system signaling circuits from the various kingdoms of life
Adrian F. Alvarez, Carlos Barba‐Ostria, Hortencia Silva‐Jiménez, Dimitris Georgellis
Environmental Microbiology.2016; 18(10): 3210. CrossRef
Effects of the Global Regulator CsrA on the BarA/UvrY Two-Component Signaling System
Martha I. Camacho, Adrian F. Alvarez, Ricardo Gonzalez Chavez, Tony Romeo, Enrique Merino, Dimitris Georgellis, J. S. Parkinson
Journal of Bacteriology.2015; 197(5): 983. CrossRef
Metabolic Engineering of Escherichia coli for Production of Mixed-Acid Fermentation End Products
Andreas H. FÃ¶rster, Johannes Gescher
Frontiers in Bioengineering and Biotechnology.2014;[Epub] CrossRef
Probing the ArcA regulon in the rumen bacterium Mannheimia succiniciproducens by genome-wide expression profiling
Seulgi Yun, Jong Moon Shin, Oh-Cheol Kim, Young Ryul Jung, Doo-Byoung Oh, Sang Yup Lee, Ohsuk Kwon
Journal of Microbiology.2012; 50(4): 665. CrossRef
ArcS, the Cognate Sensor Kinase in an Atypical Arc System of Shewanella oneidensis MR-1
Jürgen Lassak, Anna-Lena Henche, Lucas Binnenkade, Kai M. Thormann
Applied and Environmental Microbiology.2010; 76(10): 3263. CrossRef
The Physiological Stimulus for the BarA Sensor Kinase
Ricardo Gonzalez Chavez, Adrian F. Alvarez, Tony Romeo, Dimitris Georgellis
Journal of Bacteriology.2010; 192(7): 2009. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

NOTE] Evidence Against the Physiological Role of Acetyl Phosphate in the Phosphorylation of the ArcA Response Regulator in Escherichia coli

J. Microbiol. 2009;47(5):657-662. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-009-0087-9

Related articles