Identification of the Vibrio vulnificus ahpC1 Gene and Its Influence on Survival under Oxidative Stress and Virulence

Woon Ki Baek; Hyun Sung Lee; Man Hwan Oh; Myung Jin Koh; Kun-Soo Kim; Sang Ho Choi

doi:10.1007/s12275-009-0130-x

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Identification of the Vibrio vulnificus ahpC1 Gene and Its Influence on Survival under Oxidative Stress and Virulence: Woon Ki Baek ¹, Hyun Sung Lee ¹, Man Hwan Oh ¹, Myung Jin Koh ¹, Kun-Soo Kim ², Sang Ho Choi ¹; Journal of Microbiology 2009;47(5):624-632.
DOI: https://doi.org/10.1007/s12275-009-0130-x
Published online: October 24, 2009

¹National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, ²Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Republic of Korea¹National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea, ²Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Republic of Korea

Corresponding author: Sang Ho Choi , Tel: 82-2-880-4857,

Received: 22 April 2009 • Accepted: 27 May 2009

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Abstract

Pathogens have evolved sophisticated mechanisms to survive oxidative stresses imposed by host defense systems, and the mechanisms are closely linked to their virulence. In the present study, ahpC1, a homologue of Escherichia coli ahpC encoding a peroxiredoxin, was identified among the Vibrio vulnificus genes specifically induced by exposure to H2O2. In order to analyze the role of AhpC1 in the pathogenesis of V. vulnificus, a mutant, in which the ahpC1 gene was disrupted, was constructed by allelic exchanges. The ahpC1 mutant was hypersusceptable to killing by reactive oxygen species (ROS) such as H2O2 and t-BOOH, which is one of the most commonly used hydroperoxides in vitro. The purified AhpC1 reduced H2O2 in the presence of AhpF and NADH as a hydrogen donor, indicating that V. vulnificus AhpC1 is a NADH-dependent peroxiredoxin and constitutes a peroxide reductase system with AhpF. Compared to wild type, the ahpC1 mutant exhibited less cytotoxicity toward INT-407 epithelial cells in vitro and reduced virulence in a mouse model. In addition, the ahpC1 mutant was significantly diminished in growth with INT-407 epithelial cells, reflecting that the ability of the mutant to grow, survive, and persist during infection is also impaired. Consequently, the combined results suggest that AhpC1 and the capability of resistance to oxidative stresses contribute to the virulence of V. vulnificus by assuring growth and survival during infection.

Keywords: V. vulnificus ahpC1;oxidative stress;virulence

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Identification of the Vibrio vulnificus ahpC1 Gene and Its Influence on Survival under Oxidative Stress and Virulence

J. Microbiol. 2009;47(5):624-632. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-009-0130-x

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