Identification and Characterization of a Novel Antibacterial Peptide, Avian β-Defensin 2 from Ducks

Deying Ma; Ruiqin Wang; Wenyan Liao; Zongxi Han; Shengwang Liu

doi:10.1007/s12275-009-0068-z

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Identification and Characterization of a Novel Antibacterial Peptide, Avian β-Defensin 2 from Ducks: Deying Ma ¹, Ruiqin Wang ^1,2, Wenyan Liao ^1,2, Zongxi Han ², Shengwang Liu ²; Journal of Microbiology 2009;47(5):610-618.
DOI: https://doi.org/10.1007/s12275-009-0068-z
Published online: October 24, 2009

¹Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, P. R. China, ²Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P. R. China¹Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, P. R. China, ²Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P. R. China

Corresponding author: Shengwang Liu , Tel: 86-451-8593-5065,

Received: 9 March 2009 • Accepted: 20 May 2009

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Abstract

In this study, a novel avian β-defensin (AvBD) was isolated from duck pancreas. The complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. Homology, characterization and comparison of the gene with AvBD from other avian species confirmed that it was duck AvBD2. The mRNA expression of the gene was analyzed in 17 tissues from 21-day-old ducks. AvBD2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed in the muscular stomach, small intestine, kidney, spleen, thymus, and bursa of Fabricius; and weakly expressed in skin. We produced and purified recombinant AvBD2 by expressing the gene in Escherichia coli. As expected, the recombinant peptide exhibited strong bactericidal properties against Bacillus cereus, Staphylococcus aureus, and Pasteurella multocida, and weak bactericidal properties against E. coli and Salmonella choleraesuis. In addition, the recombinant protein retained antimicrobial activity against S. aureus under different temperatures (range, -20°C to 100°C) and pH values (range, 3 to 12).

Keywords: duck;AvBD;recombinant protein;antimicrobial activity

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Identification and Characterization of a Novel Antibacterial Peptide, Avian β-Defensin 2 from Ducks

J. Microbiol. 2009;47(5):610-618. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-009-0068-z

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