Response Surface Analysis for the Production of an Enantioselective Lipase from Aspergillus niger by Solid-State Fermentation

Fabiano Jares Contesini; Vania Castriani Fernades da Silva; Rafael Ferreira Maciel; Rosemary Joana de Lima; Francisco Fábio Cavalcante Barros; Patrícia de Oliveira Carvalho

doi:10.1007/s12275-008-0279-8

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Response Surface Analysis for the Production of an Enantioselective Lipase from Aspergillus niger by Solid-State Fermentation: Fabiano Jares Contesini ¹, Vania Castriani Fernades da Silva ², Rafael Ferreira Maciel ², Rosemary Joana de Lima ², Francisco Fábio Cavalcante Barros ¹, Patrícia de Oliveira Carvalho ²; Journal of Microbiology 2009;47(5):563-571.
DOI: https://doi.org/10.1007/s12275-008-0279-8
Published online: October 24, 2009

¹Laboratório de Bioquímica de Alimentos, Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas (UNICAMP) 13083-862, Brazil, ²Curso de Farmácia, Universidade São Francisco 12916-900, Brazil

Corresponding author: Fabiano Jares Contesini , Tel: 55-19-3521-2175,

Received: 19 November 2008 • Accepted: 21 June 2009

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Abstract

The lipase produced by the Aspergillus niger strain AC-54 has been widely studied due to its enantioselectivity for racemic mixtures. This study aimed to optimize the production of this enzyme using statistical methodology. Initially a Plackett-Burman (PB) design was used to evaluate the effects of the culture medium components and the culture conditions. Twelve factors were screened: water content, glucose, yeast extract, peptone, olive oil, temperature, NaH2PO4, KH2PO4, MgSO4․7H2O, CaCl2, NaCl, and MnSO4. The screening showed that the significant factors were water content, glucose, yeast extract, peptone, NaH2PO4, and KH2PO4, which were optimized using response surface methodology (RSM) and a mathematical model obtained to explain the behavioral process. The best lipase activity was attained using the following conditions: water content (20%), glucose (4.8%), yeast extract (4.0%), and NaH2PO4 (4.0%). The predicted lipase activity was 33.03 U/ml and the experimental data confirmed the validity of the model. The enzymatic activity was expressed as µmoles of oleic acid released per minute of reaction (µmol/min).

Keywords: lipase;response surface methodology;A. niger

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Response Surface Analysis for the Production of an Enantioselective Lipase from Aspergillus niger by Solid-State Fermentation

J. Microbiol. 2009;47(5):563-571. Published online October 24, 2009

DOI: https://doi.org/10.1007/s12275-008-0279-8

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