Analysis of a Novel Class 1 Integron Containing Metallo-β-Lactamase Gene VIM-2 in Pseudomonas aeruginosa

Jae Hoon Jeong; Kyeong Seob Shin; Jang Won Lee; Eun Jin Park; Seung-Yeol Son

doi:10.1007/s12275-008-0272-2

Articles

Page Path: HOME > J. Microbiol > Volume 47(6); 2009 > Article

Research Support, Non-U.S. Gov't
Analysis of a Novel Class 1 Integron Containing Metallo-β-Lactamase Gene VIM-2 in Pseudomonas aeruginosa: Jae Hoon Jeong ¹, Kyeong Seob Shin ², Jang Won Lee ¹, Eun Jin Park ¹, Seung-Yeol Son ¹; Journal of Microbiology 2009;47(6):753-759.
DOI: https://doi.org/10.1007/s12275-008-0272-2
Published online: February 4, 2010

¹Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan 330-714, Republic of Korea, ²Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea¹Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan 330-714, Republic of Korea, ²Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea

Corresponding author: Seung-Yeol Son , Tel: 82-41-550-3455,

Received: 11 November 2008 • Accepted: 13 February 2009

prev next

58 Views
0 Download
31 Crossref
34 Scopus

Full Article

Abstract

Carbapenems such as imipenem are stable to most β-lactamases. Recently, increased numbers of carbapenemase producing Gram-negative bacterial strains have been isolated because of the increased use of cabapenems. In this respect, control of these infectious carbapenemase producing Gram-negative bacteria and understanding their resistance mechanism are becoming more important. These carbapenem-hydrolyzing β-lactamase genes have been reported to exist mostly as gene cassettes in an integron. This implies that antibiotic resistance genes may be transferred to other bacteria via the integron. In the present study, we identified and analyzed an integron containing VIM-2 type metallo-β-lactamase gene in a carbapenemase producing Pseudomonas aeruginosa. In addition, the possibility of resistance spread by integron located in a plasmid was tested. Among glucose non-fermenting Gram-negative bacilli with reduced imipenem susceptibility (MIC≥8 μg/ml) isolated from Korean patients, P. aeruginosa 1082 showed resistance to most β-lactams, cephalosporin, and aminoglycoside. We found that P. aeruginosa 1082 was inhibited by EDTA in EDTA double disk synergy test which means that this strain produces metallo-β-lactamase. Class 1 integron containing blaVIM-2 (carbapenem resistance gene), qacF (quaternary ammonium compound resistance gene), aacA4 (aminoglycoside resistance gene), catB3 (chloramphenicol resistance gene), blaOXA-30 (extended-spectrum β-lactam resistance gene), and aadA1 (aminoglycoside resistance gene) gene cassettes was detected in P. aeruginosa 1082. The size of the integron was 5,246 bp and the structure and arrangement of the integron was a novel one in comparison with other integrons found in other P. aeruginosa. The integron could be transferred to Escherichia coli JM109 from P. aeruginosa 1082 possibly via self-transferable plasmid DNA. The integron and a blaVIM-2 gene were detected in the plasmid DNA of the transconjugants whose imipenem resistance was slightly increased as a result of accepting the integron from the donor strain.

Keywords: blaVIM-2;Class 1 integron;P. aeruginosa

Figure & Data

References

Citations

Citations to this article as recorded by

Difficult-to-treat (DTR) Pseudomonas aeruginosa harboring Verona-Integron metallo-β-lactamase ( bla VIM ): infection management and molecular analysis
Ana D. Vega, Kailynn DeRonde, Adriana Jimenez, Michael Piazza, Christine Vu, Octavio Martinez, Laura J. Rojas, Steven Marshall, Mohamad Yasmin, Robert A. Bonomo, Lilian M. Abbo, Pranita D. Tamma
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Prioritization of Critical Factors for Surveillance of the Dissemination of Antibiotic Resistance in Pseudomonas aeruginosa: A Systematic Review
Jung Hun Lee, Nam-Hoon Kim, Kyung-Min Jang, Hyeonku Jin, Kyoungmin Shin, Byeong Chul Jeong, Dae-Wi Kim, Sang Hee Lee
International Journal of Molecular Sciences.2023; 24(20): 15209. CrossRef
Facile Synthesis of chitosan-g-PVP/f-MWCNTs for application in Cu(II) ions removal and for bacterial growth inhibition in aqueous solutions
Samira T. Rabie, Yasser Mahmoud A. Mohamed, Reham A. Abdel-Monem, Hossam A. El Nazer
Scientific Reports.2022;[Epub] CrossRef
Genetic Diversity, Distribution, and Genomic Characterization of Antibiotic Resistance and Virulence of Clinical Pseudomonas aeruginosa Strains in Kenya
Shahiid Kiyaga, Cecilia Kyany'a, Angela W. Muraya, Hunter J. Smith, Emma G. Mills, Caleb Kibet, Gerald Mboowa, Lillian Musila
Frontiers in Microbiology.2022;[Epub] CrossRef
Pseudomonas aeruginosa efflux pump superfamily (review of literature)
Mikhail Eduardovich Ivanov, N. K. Fursova, V. D. Potapov
Russian Clinical Laboratory Diagnostics.2022; 67(1): 53. CrossRef
Mechanical, thermal and antibacterial performances of acrylonitrile butadiene rubber/polyvinyl chloride loaded with Moringa oleifera leaves powder
Ahmed M. Khalil, Samira T. Rabie
Journal of Thermal Analysis and Calorimetry.2021; 143(4): 2973. CrossRef
Promising features for poly(vinyl chloride) enriched with Moringa oleifera: Photostability, rheological, mechanical, thermal and antibacterial properties
Ahmed M. Khalil, Reham A. Abdel‐Monem, Samira T. Rabie
Journal of Vinyl and Additive Technology.2021; 27(1): 28. CrossRef
Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
Marco A. Brovedan, Patricia M. Marchiaro, María S. Díaz, Diego Faccone, Alejandra Corso, Fernando Pasteran, Alejandro M. Viale, Adriana S. Limansky
Infection, Genetics and Evolution.2021; 96: 105131. CrossRef
Comparative Evaluation of the Effects of Binzalkonium Chloride, Iodine, Gluteraldehyde and Hydrogen Peroxide Disinfectants against Avian Salmonellae Focusing on Genotypic Resistance Pattern of the Salmonellae Serotypes toward Benzalkonium Chloride
A Aksoy, KEM El Kahlout, H Yardimci
Brazilian Journal of Poultry Science.2020;[Epub] CrossRef
Thiazole derivatives‐functionalized polyvinyl chloride nanocomposites with photostability and antimicrobial properties
Samir T. Gaballah, Ahmed M. Khalil, Samira T. Rabie
Journal of Vinyl and Additive Technology.2019;[Epub] CrossRef
Molecular Analysis of Carbapenem-Resistant Pseudomonas aeruginosa Isolated from Patients Hospitalized in Daejeon between 2008 and 2014 Years
Hye Hyun Cho
The Korean Journal of Clinical Laboratory Science.2018; 50(4): 406. CrossRef
Antimicrobial activity of PVC-pyrazolone-silver nanocomposites
Ahmed A. El-Sayed, Ahmed M. Khalil, Mahmoud El-Shahat, Nahid Y. Khaireldin, Samira T. Rabie
Journal of Macromolecular Science, Part A.2016; 53(6): 346. CrossRef
Antibiotic Resistant Bacteria Found in Municipal Drinking Water
Sadia Khan, Charles W. Knapp, Tara K. Beattie
Environmental Processes.2016; 3(3): 541. CrossRef
Pseudomonas aeruginosa and Achromobacter sp. Clonal Selection Leads to Successive Waves of Contamination of Water in Dental Care Units
Fatima Abdouchakour, Chloé Dupont, Delphine Grau, Fabien Aujoulat, Patricia Mournetas, Hélène Marchandin, Sylvie Parer, Philippe Gibert, Jean Valcarcel, Estelle Jumas-Bilak, J. L. Schottel
Applied and Environmental Microbiology.2015; 81(21): 7509. CrossRef
A fully sealed plastic chip for multiplex PCR and its application in bacteria identification
Youchun Xu, He Yan, Yan Zhang, Kewei Jiang, Ying Lu, Yonghong Ren, Hui Wang, Shan Wang, Wanli Xing
Lab on a Chip.2015; 15(13): 2826. CrossRef
Oral Gram-negative anaerobic bacilli as a reservoir of β-lactam resistance genes facilitating infections with multiresistant bacteria
Clarisse Dupin, Zohreh Tamanai-Shacoori, Elodie Ehrmann, Anais Dupont, Frédérique Barloy-Hubler, Latifa Bousarghin, Martine Bonnaure-Mallet, Anne Jolivet-Gougeon
International Journal of Antimicrobial Agents.2015; 45(2): 99. CrossRef
Detection and characterization of class 1 integron-associated gene cassettes from Pseudomonas aeruginosa isolates in southern Taiwan
Ke-Yu Hsiao, Mei-Feng Lee, Chien-Fang Peng
Biomarkers and Genomic Medicine.2014; 6(2): 74. CrossRef
Dissemination of IMP-6-producing Pseudomonas aeruginosa ST244 in multiple cities in China
Y. Chen, M. Sun, M. Wang, Y. Lu, Z. Yan
European Journal of Clinical Microbiology & Infectious Diseases.2014; 33(7): 1181. CrossRef
Characterization of Carbapenem Nonsusceptible Pseudomonas aeruginosa in Denmark: A Nationwide, Prospective Study
Frank Hansen, Helle Krogh Johansen, Claus Østergaard, Magnus Arpi, Dennis Schrøder Hansen, Pia Littauer, Anette Holm, Ole Heltberg, Helga Schumacher, Kurt Fuursted, Mari-Ann Domar Lykke, Birgitte Tønning, Anette M. Hammerum, Ulrik Stenz Justesen
Microbial Drug Resistance.2014; 20(1): 22. CrossRef
Molecular Characterization of Carbapenem-Resistant Strains ofKlebsiella pneumoniaeIsolated from Iranian Patients: First Identification ofblaKPCGene in Iran
Saman Nobari, Fereshteh Shahcheraghi, Fatemeh Rahmati Ghezelgeh, Babak Valizadeh
Microbial Drug Resistance.2014; 20(4): 285. CrossRef
Prevalence of bacteria resistant to antibiotics and/or biocides on meat processing plant surfaces throughout meat chain production
Leyre Lavilla Lerma, Nabil Benomar, Antonio Gálvez, Hikmate Abriouel
International Journal of Food Microbiology.2013; 161(2): 97. CrossRef
Carbapenemases: Partners in crime
Karen Bush
Journal of Global Antimicrobial Resistance.2013; 1(1): 7. CrossRef
New integron gene arrays from multiresistant clinical isolates of members of the Enterobacteriaceae and Pseudomonas aeruginosa from hospitals in Malaysia
Sue-Bee Kor, Quok-Cheong Choo, Choy-Hoong Chew
Journal of Medical Microbiology .2013; 62(3): 412. CrossRef
Analysis of integrons and associated gene cassettes in clinical isolates of multidrug resistant Pseudomonas aeruginosa from Southwest Nigeria
Bamidele T Odumosu, Bolanle A Adeniyi, Ram Chandra
Annals of Clinical Microbiology and Antimicrobials.2013; 12(1): 29. CrossRef
Emergence of resistance to antibacterial agents: the role of quaternary ammonium compounds—a critical review
Sylvie Buffet-Bataillon, Pierre Tattevin, Martine Bonnaure-Mallet, Anne Jolivet-Gougeon
International Journal of Antimicrobial Agents.2012; 39(5): 381. CrossRef
Genetic basis of resistance to quaternary ammonium compounds - the qac genes and their role: a review
Z. Jaglic, D. Cervinkova
Veterinární medicína.2012; 57(6): 275. CrossRef
Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia
Sanda Sardelic, Branka Bedenic, Céline Colinon-Dupuich, Stjepan Orhanovic, Zrinka Bosnjak, Vanda Plecko, Benoit Cournoyer, Gian Maria Rossolini
Antimicrobial Agents and Chemotherapy.2012; 56(5): 2746. CrossRef
Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia
Yalda Khosravi, Sun Tee Tay, Jamuna Vadivelu
Journal of Medical Microbiology.2011; 60(7): 988. CrossRef
Résistance aux carbapénèmes : vers une nouvelle impasse ?
N. Grall, A. Andremont, L. Armand-Lefèvre
Journal des Anti-infectieux.2011; 13(2): 87. CrossRef
Multidrug-resistant Organisms and Healthcare-associated Infections
Mi-Na Kim
Hanyang Medical Reviews.2011; 31(3): 141. CrossRef
The Accessory Genome of Pseudomonas aeruginosa
Vanderlene L. Kung, Egon A. Ozer, Alan R. Hauser
Microbiology and Molecular Biology Reviews.2010; 74(4): 621. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Analysis of a Novel Class 1 Integron Containing Metallo-β-Lactamase Gene VIM-2 in Pseudomonas aeruginosa

J. Microbiol. 2009;47(6):753-759. Published online February 4, 2010

DOI: https://doi.org/10.1007/s12275-008-0272-2

Related articles