Isolation, Characterization, and Evaluation of Wild Isolates of Lactobacillus reuteri from Pig Feces

Deog Yong Lee; Yeon-Soo Seo; Nabin Rayamajhi; Mi Lan Kang; Su In Lee; Han Sang Yoo

doi:10.1007/s12275-009-0124-8

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Isolation, Characterization, and Evaluation of Wild Isolates of Lactobacillus reuteri from Pig Feces: Deog Yong Lee , Yeon-Soo Seo , Nabin Rayamajhi , Mi Lan Kang , Su In Lee , Han Sang Yoo; Journal of Microbiology 2009;47(6):663-672.
DOI: https://doi.org/10.1007/s12275-009-0124-8
Published online: February 4, 2010

Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Priority Research Institute and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea

Corresponding author: Han Sang Yoo , Tel: 82-2-880-1263,

Received: 19 April 2009 • Accepted: 26 June 2009

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Abstract

Lactic acid bacteria (LAB) are a well-used probiotics for health improvements in both humans and animals. Despite of several benefits, non-host-specific LAB showed poor probiotics effects due to difficulty in colonization and competition with normal flora. Therefore, the feasibility of porcine LAB isolates was evaluated as a probiotics. Ten of 49 Lactobacillus spp. isolates harbored 2~10 kb plasmid DNA. Seven strains were selected based on the safety test, such as hemolytic activity, ammonia, indole, and phenylalanine production. After safety test, five strains were selected again by several tests, such as epithelial adherence, antimicrobial activity, tolerance against acid, bile, heat, and cold-drying, and production of acid and hydrogen peroxide. Then, enzyme profiles (ZYM test) and antibiotics resistance were analyzed for further characterization. Five Lactobacillus reuteri isolates from pig feces were selected by safety and functional tests. The plasmid DNA which was able to develop vector system was detected in the isolates. Together with these approaches, pig-specific Lactobacillus spp. originated from pigs were selected. These strains may be useful tools to develop oral delivery system.

Keywords: probiotics;Lactobacillus spp.;safety;characteristics;tolerance

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Isolation, Characterization, and Evaluation of Wild Isolates of Lactobacillus reuteri from Pig Feces

J. Microbiol. 2009;47(6):663-672. Published online February 4, 2010

DOI: https://doi.org/10.1007/s12275-009-0124-8

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