Cotesia plutellae Bracovirus Suppresses Expression of an Antimicrobial Peptide, Cecropin, in the Diamondback Moth, Plutella xylostella, Challenged by Bacteria

Karen P. Barandoc; Jaehyun Kim; Yonggyun Kim

doi:10.1007/s12275-009-9261-3

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Cotesia plutellae Bracovirus Suppresses Expression of an Antimicrobial Peptide, Cecropin, in the Diamondback Moth, Plutella xylostella, Challenged by Bacteria: Karen P. Barandoc , Jaehyun Kim , Yonggyun Kim; Journal of Microbiology 2010;48(1):117-123.
DOI: https://doi.org/10.1007/s12275-009-9261-3
Published online: March 11, 2010

Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of KoreaDepartment of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea

Corresponding author: Yonggyun Kim , Tel: +82-54-820-5638,

Received: 18 August 2008 • Accepted: 18 September 2009

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Abstract

An endoparasitoid wasp, Cotesia plutellae, induces significant immunosuppression of host insect, Plutella xylostella. This study was focused on suppression in humoral immune response of P. xylostella parasitized by C. plutellae. An EST database of P. xylostella provided a putative cecropin gene (PxCec) which is 627 bp long and encodes 66 amino acids. A signal peptide (22 amino acids) is predicted and two putative O-glycosylation sites in threonine are located at positions 58 and 64. Without bacterial infection, PxCec was expressed in pupa and adult stages but not in the egg and larval stages. Upon bacterial challenge, however, the larvae expressed PxCec as early as 3 h post infection (PI) and maintained high expression levels at 12-24 h PI. By 48 h PI, its expression noticeably diminished. All tested tissues of bacteria-infected P. xylostella showed PxCec expression. However, other microbes, such as virus and fungus, did not induce the PxCec expression. Parasitization by C. plutellae suppressed the expression of PxCec in response to bacterial challenge. Among the parasitic factors of C. plutellae, its symbiotic virus (C. plutellae bracovirus: CpBV) alone was able to inhibit the expression of PxCec of P. xylostella challenged by bacteria. These results indicate that PxCec expression is regulated by both immune and developmental processes in P. xylostella. The parasitization by C. plutellae inhibited the expression of PxCec by the wasp’s symbiotic virus.

Keywords: polydnavirus;cecropin;P. xylostella;C. plutellae;expression;immunosuppression

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Cotesia plutellae Bracovirus Suppresses Expression of an Antimicrobial Peptide, Cecropin, in the Diamondback Moth, Plutella xylostella, Challenged by Bacteria

J. Microbiol. 2010;48(1):117-123. Published online March 11, 2010

DOI: https://doi.org/10.1007/s12275-009-9261-3

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