ppGpp-Mediated Stationary Phase Induction of the Genes Encoded by Horizontally Acquired Pathogenicity Islands and cob/pdu Locus in Salmonella enterica serovar Typhimurium

Miryoung Song; Hyun-Ju Kim; Sangryeol Ryu; Hyunjin Yoon; Jiae Yun; Hyon E. Choy

doi:10.1007/s12275-009-0179-6

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ppGpp-Mediated Stationary Phase Induction of the Genes Encoded by Horizontally Acquired Pathogenicity Islands and cob/pdu Locus in Salmonella enterica serovar Typhimurium: Miryoung Song ^1,2, Hyun-Ju Kim ^1,2, Sangryeol Ryu ³, Hyunjin Yoon ³, Jiae Yun ³, Hyon E. Choy ^1,2; Journal of Microbiology 2010;48(1):89-95.
DOI: https://doi.org/10.1007/s12275-009-0179-6
Published online: March 11, 2010

¹Research Center for Enteropathogenic Bacteria and Research Institute of Vibrio Infection, Chonnam National University Medical College, Gwangju 501-746, Republic of Korea, ²Department of Microbiology, Chonnam National University Medical College, Gwangju 501-746, Republic of Korea, ³Department of Food and Animal Technology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Republic of Korea¹Research Center for Enteropathogenic Bacteria and Research Institute of Vibrio Infection, Chonnam National University Medical College, Gwangju 501-746, Republic of Korea, ²Department of Microbiology, Chonnam National University Medical College, Gwangju 501-746, Republic of Korea, ³Department of Food and Animal Technology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, Seoul 151-742, Republic of Korea

Corresponding author: Hyon E. Choy , Tel: +82-62-220-4137,

Received: 6 June 2009 • Accepted: 14 August 2009

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Abstract

Salmonella enterica is highly diverse in terms of genome structure, which is at least partly due to the horizontal transfer of genetic elements from various sources. In this study, we examined the expression profiles of such genes in Salmonella Pathogenicity Islands (SPIs) and the cob/pdu locus, horizontally acquired large DNA segments, during growth under standard growth conditions. Transcripts from exponentially growing and early stationary phase Salmonellae were compared using various methods including cDNA microarray analysis. Nearly all genes encoded by SPIs and the cob/pdu locus were induced at the onset of the stationary phase in a stringent molecule ppGpp-dependent but stationary phase σ, σ38-independent manner. Although, it has been suggested that ppGpp acts in concert with DksA, we found the stationary phase induction of those SPI genes was not DksA dependent. It is suggested that ppGpp stimulates the expression of these stress-inducible genes encoded by horizontally acquired DNA, by itself or in concert with DksA.

Keywords: Salmonella;ppGpp;microarray analysis;stationary phase;pathogenic genes;dksA

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ppGpp-Mediated Stationary Phase Induction of the Genes Encoded by Horizontally Acquired Pathogenicity Islands and cob/pdu Locus in Salmonella enterica serovar Typhimurium

J. Microbiol. 2010;48(1):89-95. Published online March 11, 2010

DOI: https://doi.org/10.1007/s12275-009-0179-6

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