Antimicrobial Resistance Patterns and Characterization of Integrons of Shigella sonnei Isolates in Seoul, 1999-2008

Young-hee Jin; Young-hee Oh; Ji-hun Jung; Soo-jin Kim; Jin-ah Kim; Ki-young Han; Min-young Kim; Seog-gee Park; Young-ki Lee

doi:10.1007/s12275-010-9220-z

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Antimicrobial Resistance Patterns and Characterization of Integrons of Shigella sonnei Isolates in Seoul, 1999-2008: Young-hee Jin ¹, Young-hee Oh ¹, Ji-hun Jung ¹, Soo-jin Kim ¹, Jin-ah Kim ¹, Ki-young Han ¹, Min-young Kim ¹, Seog-gee Park ¹, Young-ki Lee ²; Journal of Microbiology 2010;48(2):236-242.
DOI: https://doi.org/10.1007/s12275-010-9220-z
Published online: May 1, 2010

¹Division of Epidemiology, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul 137-134, Republic of Korea, ²Department of Microbiology, School of Dentistry, Department of Public Health, Graduate School of Public Health & Social Welfare, Dankook University, Cheonan 330-716, Republic of Korea

Corresponding author: Young-ki Lee , Tel: +82-41-550-1998,

Received: 20 July 2009 • Accepted: 8 November 2009

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Abstract

A total of 66 Shigella sonnei isolates from 1999 to 2008 in Seoul was analyzed for their antimicrobial resistance, carriage of integron, and the patterns of Pulsed-field gel electrophoresis (PFGE). A high level of antimicrobial resistance to streptomycin (100%), trimethoprim/sulfamethoxazole (95%), tetracycline (94%), nalidixic acid (65%), and ampicillin (41%) was observed among S. sonnei isolates. Fourteen profiles of antimicrobial resistance were identified with the most common resistance profile being nalidixic acid, streptomycin, tetracycline, and trimethoprim/sulfamethoxazole (35%). PCR and DNA sequencing analysis revealed the presence of class 2 integron in all isolates, and class 1 and 2 integrons in 7 isolates. The class 2 integron carried two types of gene cassettes. One cassette array was dfrI, sat2, and aadA1 (91%), and the other was dfr1 and sat1 (8%). dfrA12 and aadA2 gene cassette was found in one isolate containing class 1 integron. PFGE was carried out to examine the genetic relatedness among isolates. All isolates except for one showed similar PFGE patterns (similarity of 80.1%). These results suggest that the S. sonnei isolated during 1999-2008 in Seoul have similar lineages that have not undergone evolutionary changes with time.

Keywords: S. sonnei;antimicrobial resistance;integron;PFGE

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Antimicrobial Resistance Patterns and Characterization of Integrons of Shigella sonnei Isolates in Seoul, 1999-2008

J. Microbiol. 2010;48(2):236-242. Published online May 1, 2010

DOI: https://doi.org/10.1007/s12275-010-9220-z

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