Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414

Li Wang Wang; Xiao-Fei Liu; Shi Yun; Xiao-Peng Yuan; Xu-Hu Mao; Chao Wu; Wei-Jun Zhang; Kai-Yun Liu; Gang Guo; Dong-Shui Lu; Wen-De Tong; Ai-Dong Wen; Quan-Ming Zou

doi:10.1007/s12275-009-0233-4

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Research Support, Non-U.S. Gov't
Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414: Li Wang Wang ¹, Xiao-Fei Liu ², Shi Yun ², Xiao-Peng Yuan ^2,3, Xu-Hu Mao ², Chao Wu ², Wei-Jun Zhang ², Kai-Yun Liu ², Gang Guo ², Dong-Shui Lu ², Wen-De Tong ², Ai-Dong Wen ¹, Quan-Ming Zou ¹; Journal of Microbiology 2010;48(2):223-228
DOI: https://doi.org/10.1007/s12275-009-0233-4
Published online: May 1, 2010

¹Department of Pharmacy, Xijing Hospital, the Fourth Military Medical University, Xi`an 710032, P. R. China, ²Department of Clinical Microbiology and Immunology, School of Medical Laboratory, the Third Military Medical University, Chongqing 400038, P. R. China, ³Department of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, P. R. China¹Department of Pharmacy, Xijing Hospital, the Fourth Military Medical University, Xi`an 710032, P. R. China, ²Department of Clinical Microbiology and Immunology, School of Medical Laboratory, the Third Military Medical University, Chongqing 400038, P. R. China, ³Department of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, P. R. China

Corresponding author: Ai-Dong Wen , Tel: +86-23-6875-2316,
Quan-Ming Zou , Tel: +86-23-6875-2316,

Received: 4 August 2009 • Accepted: 27 September 2009

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Abstract

A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.

Keywords: H. pylori; multivalent vaccine; urease B subunit; H. pylori adhesin A; heat shock protein A

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Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414

J. Microbiol. 2010;48(2):223-228. Published online May 1, 2010

DOI: https://doi.org/10.1007/s12275-009-0233-4

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