Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414

Li Wang Wang; Xiao-Fei Liu; Shi Yun; Xiao-Peng Yuan; Xu-Hu Mao; Chao Wu; Wei-Jun Zhang; Kai-Yun Liu; Gang Guo; Dong-Shui Lu; Wen-De Tong; Ai-Dong Wen; Quan-Ming Zou

doi:10.1007/s12275-009-0233-4

Articles

Page Path: HOME > J. Microbiol > Volume 48(2); 2010 > Article

Research Support, Non-U.S. Gov't
Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414: Li Wang Wang ¹, Xiao-Fei Liu ², Shi Yun ², Xiao-Peng Yuan ^2,3, Xu-Hu Mao ², Chao Wu ², Wei-Jun Zhang ², Kai-Yun Liu ², Gang Guo ², Dong-Shui Lu ², Wen-De Tong ², Ai-Dong Wen ¹, Quan-Ming Zou ¹; Journal of Microbiology 2010;48(2):223-228.
DOI: https://doi.org/10.1007/s12275-009-0233-4
Published online: May 1, 2010

¹Department of Pharmacy, Xijing Hospital, the Fourth Military Medical University, Xi`an 710032, P. R. China, ²Department of Clinical Microbiology and Immunology, School of Medical Laboratory, the Third Military Medical University, Chongqing 400038, P. R. China, ³Department of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, P. R. China

Corresponding author: Ai-Dong Wen , Tel: +86-23-6875-2316,
Quan-Ming Zou , Tel: +86-23-6875-2316,

Received: 4 August 2009 • Accepted: 27 September 2009

prev next

95 Views
0 Download
10 Crossref
14 Scopus

Full Article

Abstract

A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.

Keywords: H. pylori;multivalent vaccine;urease B subunit;H. pylori adhesin A;heat shock protein A

Figure & Data

References

Citations

Citations to this article as recorded by

WITHDRAWN: PIRES2-EGFP/CTB-UreI vaccination activated a mixed Th1/Th2/Th17 immune system defense towards Helicobacter pylori infection in the BALB/c mice model
Sana Ghasemifar, Omid Chabak, Tohid Piri-Gharaghie, Abbas Doosti
Vaccine.2024; : 125733. CrossRef
Potential antigen candidates for subunit vaccine development against Helicobacter pylori infection
Masoud Keikha, Majid Eslami, Bahman Yousefi, Abdolmajid Ghasemian, Mohsen Karbalaei
Journal of Cellular Physiology.2019; 234(12): 21460. CrossRef
A novel design of a multi-antigenic, multistage and multi-epitope vaccine against Helicobacter pylori: An in silico approach
Beatriz Meza, Felipe Ascencio, Arturo Pedro Sierra-Beltrán, Javier Torres, Carlos Angulo
Infection, Genetics and Evolution.2017; 49: 309. CrossRef
The C-Terminal Disulfide Bonds of Helicobacter pylori GroES Are Critical for IL-8 Secretion via the TLR4-Dependent Pathway in Gastric Epithelial Cells
Yu-Lin Su, Jyh-Chin Yang, Haur Lee, Fuu Sheu, Chun-Hua Hsu, Shuei-Liong Lin, Lu-Ping Chow
The Journal of Immunology.2015; 194(8): 3997. CrossRef
Protection against Helicobacter pylori infection in BALB/c mice by oral administration of multi-epitope vaccine of CTB-UreI-UreB
Jing Yang, Lv-xia Dai, Xing Pan, Hongren Wang, Bei Li, Jie Zhu, Ming-yuan Li, Xin-li Shi, Bao-ning Wang
Pathogens and Disease.2015;[Epub] CrossRef
Immunology and vaccines and nanovaccines forHelicobacter pyloriinfection
Morteza Milani, Yaeghob Sharifi, Mohammad Rahmati-Yamchi, Mohammad H Somi, Abolfazl Akbarzadeh
Expert Review of Vaccines.2015; 14(6): 833. CrossRef
A recombinant chimeric protein containing B chains of ricin and abrin is an effective vaccine candidate
Junhong Wang, Shan Gao, Tao Zhang, Lin Kang, Wuchun Cao, Na Xu, Wensen Liu, Jinglin Wang
Human Vaccines & Immunotherapeutics.2014; 10(4): 938. CrossRef
Protection against pneumococcal infection elicited by immunization with multiple pneumococcal heat shock proteins
Ju Cao, Xiaojiao Zhang, Yi Gong, Yuhong Zhang, Yali Cui, Xiaofei Lai, Yibing Yin, Meijuan Li, Dairong Li, Liping Zhang
Vaccine.2013; 31(35): 3564. CrossRef
Inflammation, Immunity, and Vaccine Development for Helicobacter pylori
Anne Müller, Jay V. Solnick
Helicobacter.2011; 16(s1): 26. CrossRef
Mimotopes selected with a neutralizing antibody against urease B from Helicobacter pyloriinduce enzyme inhibitory antibodies in mice upon vaccination
Yan Li, Yunshan Ning, Yundan Wang, Dandan Peng, Yaodong Jiang, Lili Zhang, Min Long, Jun Luo, Ming Li
BMC Biotechnology.2010;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414

J. Microbiol. 2010;48(2):223-228. Published online May 1, 2010

DOI: https://doi.org/10.1007/s12275-009-0233-4

Related articles