In Vivo Studies with a Candida tropicalis Isolate Exhibiting Paradoxical Growth In Vitro in the Presence of High Concentration of Caspofungin

Sedigh Bayegan; Laszlo Majoros; Gabor Kardos; Adam Kemény-Beke; Cecilia Miszti; Renato Kovacs; Rudolf Gesztelyi

doi:10.1007/s12275-010-9221-y

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In Vivo Studies with a Candida tropicalis Isolate Exhibiting Paradoxical Growth In Vitro in the Presence of High Concentration of Caspofungin: Sedigh Bayegan ¹, Laszlo Majoros ¹, Gabor Kardos ¹, Adam Kemény-Beke ², Cecilia Miszti ¹, Renato Kovacs ¹, Rudolf Gesztelyi ³; Journal of Microbiology 2010;48(2):170-173.
DOI: https://doi.org/10.1007/s12275-010-9221-y
Published online: May 1, 2010

¹Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary, ²Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary, ³Department of Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary¹Department of Medical Microbiology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary, ²Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary, ³Department of Pharmacology, Medical and Health Science Center, University of Debrecen, Debrecen H-4032, Hungary

Corresponding author: Laszlo Majoros , Tel: +36-52-255-425,

Received: 20 July 2009 • Accepted: 1 December 2009

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Abstract

We investigated the activity of caspofungin against a Candida tropicalis clinical isolate showing paradoxical growth in vitro. BALB/c mice immunosuppressed by cyclophosphamide were infected intraperitoneally using 107 CFU/mouse. Caspofungin was administered intraperitoneally once daily for 5 days or as a single dose using the following doses: 0.12, 0.25, 1, 2, 3, 5, and 15 mg/kg. The single dose of caspofungin was effective only at 5 and 15 mg/kg concentrations (100% survival). Five-day caspofungin treatment led to 100% survival at doses of 1 mg/kg or higher. Caspofungin treatment significantly decreased the number of viable yeasts in the peritoneal lavage samples as well as in the infected abscesses at doses 1, 3, 5, and 15 mg/kg caspofungin as compared to the untreated control (P<0.001 in all cases), and even to the group treated with 0.12 mg/kg caspofungin (P<0.05 in all cases). At 2 mg/kg caspofungin dose, sterilization of the internal organs was reproducibly incomplete, suggesting that the role of paradoxical growth in the late clinical failure cannot be excluded.

Keywords: caspofungin;paradoxical growth;murine;C. tropicalis

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In Vivo Studies with a Candida tropicalis Isolate Exhibiting Paradoxical Growth In Vitro in the Presence of High Concentration of Caspofungin

J. Microbiol. 2010;48(2):170-173. Published online May 1, 2010

DOI: https://doi.org/10.1007/s12275-010-9221-y

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