Phenotypic Diversity of Escherichia coli O157:H7 Strains Associated with the Plasmid O157

Ji Youn Lim; Joon Bae Hong; Haiqing Sheng; Smriti Shringi; Rajinder Kaul; Thomas E. Besser; Carolyn J. Hovde

doi:10.1007/s12275-010-9228-4

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Research Support, U.S. Gov't, Non-P.H.S.
Phenotypic Diversity of Escherichia coli O157:H7 Strains Associated with the Plasmid O157: Ji Youn Lim ¹, Joon Bae Hong ¹, Haiqing Sheng ¹, Smriti Shringi ², Rajinder Kaul ³, Thomas E. Besser ², Carolyn J. Hovde ¹; Journal of Microbiology 2010;48(3):347-357.
DOI: https://doi.org/10.1007/s12275-010-9228-4
Published online: June 23, 2010

¹Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844, USA, ²Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA, ³Department of Medicine, University of Washington Genome Center, Seattle, Washington 98195, USA

Corresponding author: Carolyn J. Hovde , Tel: +1-208-885-5906,

Received: 27 July 2009 • Accepted: 23 December 2009

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Abstract

Escherichia coli O157:H7, a food-borne pathogen, causes hemorrhagic colitis and the hemolytic-uremic syndrome. A putative virulence factor of E. coli O157:H7 is a 60-MDa plasmid (pO157) found in 99% of all clinical isolates and many bovine-derived strains. The well characterized E. coli O157:H7 Sakai strain (Sakai) and its pO157-cured derivative (Sakai-Cu) were compared for phenotypic differences. Sakai-Cu had enhanced survival in synthetic gastric fluid, did not colonize cattle as well as wild-type Sakai, and had unchanged growth rates and tolerance to salt and heat. These results are consistent with our previous findings with another E. coli O157:H7 disease outbreak isolate ATCC 43894 and its pO157-cured (43894-Cu). However, despite the essentially sequence identical pO157 in these strains, Sakai-Cu had changes in antibiotic susceptibility and motility that did not occur in the 43894-Cu strain. This unexpected result was systematically analyzed using phenotypic microarrays testing 1,920 conditions with Sakai, 43894, and the plasmid-cured mutants. The influence of the pO157 differed between strains on a wide number of growth/survival conditions. Relative expression of genes related to acid resistance (gadA, gadX, and rpoS) and flagella production (fliC and flhD) were tested using quantitative real-time PCR and gadA and rpoS expression differed between Sakai-Cu and 43894-Cu. The strain-specific differences in phenotype that resulted from the loss of essentially DNA-sequence identical pO157 were likely due to the chromosomal genetic diversity between strains. The O157:H7 serotype diversity was further highlighted by phenotypic microarray comparisons of the two outbreak strains with a genotype 6 bovine E. coli O157:H7 isolate, rarely associated with human disease.

Keywords: E. coli O157:H7;pO157;phenotype microarray;phenotypic diversity

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Phenotypic Diversity of Escherichia coli O157:H7 Strains Associated with the Plasmid O157

J. Microbiol. 2010;48(3):347-357. Published online June 23, 2010

DOI: https://doi.org/10.1007/s12275-010-9228-4

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