Oceanobacillus manasiensis sp. nov., a Moderately Halophilic Bacterium Isolated from the Salt Lakes of Xinjiang, China

Lei Wang; Wen-Yan Liu; Zhi-Jing Gu; San-Feng Chen; Su-Sheng Yang

doi:10.1007/s12275-010-0135-5

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Oceanobacillus manasiensis sp. nov., a Moderately Halophilic Bacterium Isolated from the Salt Lakes of Xinjiang, China: Lei Wang ^1,2, Wen-Yan Liu ^2,3, Zhi-Jing Gu ², San-Feng Chen ^1,2, Su-Sheng Yang ³; Journal of Microbiology 2010;48(3):312-317.
DOI: https://doi.org/10.1007/s12275-010-0135-5
Published online: June 23, 2010

¹State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P. R. China, ²Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing100193, P. R. China, ³National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing100088, P. R. China¹State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P. R. China, ²Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing100193, P. R. China, ³National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing100088, P. R. China

Corresponding author: Su-Sheng Yang , Tel: +86-10-6273-7827,

Received: 9 April 2010 • Accepted: 1 June 2010

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Abstract

Three Gram reaction positive, rod-shaped, moderately motile halophilic bacterial strains, designated YD3-56T, YD16, and YH29, were isolated from the sediments of Manasi and Aiding salt lakes in the Xinjiang region of China, respectively. The strains grew optimally at 30-37°C, pH 8-11, in the presence of 5-10% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were closely related to members of the genus Oceanobacillus, exhibiting 99.1-99.2% similarity to O. kapialis KCTC 13177T, 99.2-99.3% to O. picturae KCTC 3821T, and 94.2-96% sequence similarity to other described Oceanobacillus species. SDS-PAGE of whole cell proteins preparations demonstrated that the strains exhibited high similarity to each other, but distinguished from O. kapialis KCTC 13177T and O. picturae KCTC 3821T (75%). DNA-DNA hybridization revealed that the similarity between the representative strain YD3-56T and O. kapialis KCTC 13177T was 35.3%, and the similarity between YD3-56T and O. picturae KCTC 3821T was 22.3%. Chemotaxonomic analysis of the strains showed menaquinone-7 was the predominant respiratory quinine. Major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipid pattern for strain YD3-56T predominantly contained phosphatidylcholine, and trace to moderate amounts of phosphatidyl ethanolamine and hydroxy-phosphatidyl ethanolamine. The diamino acid in murein was meso-diaminopimelic acid. The DNA G+C content of the strains was 39.7-40.1 mol%. On the basis of these results, the three strains should be classified as a novel species of the genus Oceanobacillus, for which the name Oceanobacillus manasiensis sp. nov. has been proposed, with the type strain as YD3-56T (=CGMCC 1.9105T =NBRC 105903T).

Keywords: Oceanobacillus manasiensis sp. nov.;moderate halophile;salt lake;Gram-positive

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Oceanobacillus manasiensis sp. nov., a Moderately Halophilic Bacterium Isolated from the Salt Lakes of Xinjiang, China

J. Microbiol. 2010;48(3):312-317. Published online June 23, 2010

DOI: https://doi.org/10.1007/s12275-010-0135-5

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