Glycosylation and Production Characteristics of Epothilones in Alkali-Tolerant Sorangium cellulosum Strain So0157-2

Lin Zhao; Peng-fei Li; Chun-hua Lu; Shu-guang Li; Yue-mao Shen; Yue-zhong Li

doi:10.1007/s12275-010-0048-3

Articles

Page Path: HOME > J. Microbiol > Volume 48(4); 2010 > Article

Research Support, Non-U.S. Gov't
Glycosylation and Production Characteristics of Epothilones in Alkali-Tolerant Sorangium cellulosum Strain So0157-2: Lin Zhao ¹, Peng-fei Li ¹, Chun-hua Lu ², Shu-guang Li ¹, Yue-mao Shen ², Yue-zhong Li ¹; Journal of Microbiology 2010;48(4):438-444.
DOI: https://doi.org/10.1007/s12275-010-0048-3
Published online: August 20, 2010

¹State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, P. R. China, ²Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen Engineering Research Center of Marine Microbial Drug Discovery, Fujian Laboratory of Pharmaceutical Engineering, School of Life Science, Xiamen University, Xiamen, Fujian 361005, P. R. China¹State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, P. R. China, ²Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen Engineering Research Center of Marine Microbial Drug Discovery, Fujian Laboratory of Pharmaceutical Engineering, School of Life Science, Xiamen University, Xiamen, Fujian 361005, P. R. China

Corresponding author: Yue-mao Shen , Tel: +86-531-8856-4288,
Yue-zhong Li , Tel: +86-531-8856-4288,

Received: 2 February 2010 • Accepted: 10 April 2010

prev next

43 Views
0 Download
11 Crossref
15 Scopus

Full Article

Abstract

3-O-α-D-ribofuranosyl epothilone A (epothiloneoside A) is a major component of glycosylated epothilones in Sorangium cellulosum strain So0157-2. The production and glycosylation ratios of epothiloneoside A in both solid and liquid culture conditions with various pH values and carbon sources were studied. The results showed that glycosylation occurs whenever epothilones are produced, regardless of changes in pH values, production time curves, and different carbon sources. We suggest that glycosylation is a stable process, paralleling the biosynthesis of epothilones in the So0157-2 strain.

Keywords: S. cellulosum;epothilones;glycosylation;epothiloneoside A

Figure & Data

References

Citations

Citations to this article as recorded by

Genetic manipulation and tools in myxobacteria for the exploitation of secondary metabolism
Xinjing Yue, Duohong Sheng, Li Zhuo, Yue-Zhong Li
Engineering Microbiology.2023; 3(2): 100075. CrossRef
Phylogeny‐guided characterization of glycosyltransferases for epothilone glycosylation
Peng Zhang, Zheng Zhang, Zhi‐feng Li, Qi Chen, Yao‐yao Li, Ya Gong, Xin‐jing Yue, Duo‐hong Sheng, You‐ming Zhang, Changsheng Wu, Yue‐zhong Li
Microbial Biotechnology.2019; 12(4): 763. CrossRef
Isolation and characterisation of the epothilone gene cluster with flanks from high alkalotolerant strain Sorangium cellulosum (So0157-2)
Zhi-Feng Li, Li-Ping Zhu, Jing-yan Gu, Raghvendra Pratap Singh, Yue-Zhong Li
World Journal of Microbiology and Biotechnology.2017;[Epub] CrossRef
Highly Efficient CYP167A1 (EpoK) dependent Epothilone B Formation and Production of 7-Ketone Epothilone D as a New Epothilone Derivative
Fredy Kern, Tobias K. F. Dier, Yogan Khatri, Kerstin M. Ewen, Jean-Pierre Jacquot, Dietrich A. Volmer, Rita Bernhardt
Scientific Reports.2015;[Epub] CrossRef
Purification of Epothilones A and B with Column Chromatography on a Sephadex LH-20
Ji Kun Yang, Lin Zhao, Rui Cheng Sun, Yue Mao Shen, Nai Qiang Wang, Xin Li Liu
Advanced Materials Research.2014; 904: 164. CrossRef
Enzymatic synthesis of epothilone A glycosides
Prakash Parajuli, Ramesh Prasad Pandey, Niranjan Koirala, Yeo Joon Yoon, Byung-Gee Kim, Jae Kyung Sohng
AMB Express.2014;[Epub] CrossRef
Extraordinary expansion of a Sorangium cellulosum genome from an alkaline milieu
Kui Han, Zhi-feng Li, Ran Peng, Li-ping Zhu, Tao Zhou, Lu-guang Wang, Shu-guang Li, Xiao-bo Zhang, Wei Hu, Zhi-hong Wu, Nan Qin, Yue-zhong Li
Scientific Reports.2013;[Epub] CrossRef
Establishment of Sorangium cellulosum So0157-2 Proteome Database Using Optimized Two-dimensional Electrophoresis Protocol*
Peng-Yi ZHANG, Yue-Zhong LI, Zhi-Hong WU, Hong LIU, Pei-Pei XU, Juan XIONG
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS.2012; 39(1): 86. CrossRef
Novel Characters of Myxobacterial Modular Natural Product Assembly Lines*
Li-Ping ZHU, Zhi-Feng Li, Kui HAN, Shu-Guang LI, Yue-Zhong LI
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS.2012; 39(6): 525. CrossRef
Analysis of the Extraction Effects of Epothilones with Different Organic Solvents
Lin Zhao, Ji Lin Dai, Jia Yi Wang, Ya Wei Li, Xin Li Liu
Advanced Materials Research.2011; 343-344: 958. CrossRef
A Novel Cold-Adapted Lipase from Sorangium cellulosum Strain So0157-2: Gene Cloning, Expression, and Enzymatic Characterization
Yuan-Yuan Cheng, Yun-Kai Qian, Zhi-Feng Li, Zhi-Hong Wu, Hong Liu, Yue-Zhong Li
International Journal of Molecular Sciences.2011; 12(10): 6765. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Glycosylation and Production Characteristics of Epothilones in Alkali-Tolerant Sorangium cellulosum Strain So0157-2

J. Microbiol. 2010;48(4):438-444. Published online August 20, 2010

DOI: https://doi.org/10.1007/s12275-010-0048-3

Related articles