Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial and Marine Environments Revealed by Enrichment Culture

Eun Hye Kim; Kyeung Hee Cho; Yung Mi Lee; Joung Han Yim; Hong Kum Lee; Jang-Cheon Cho; Soon Gyu Hong

doi:10.1007/s12275-010-0015-z

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Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial and Marine Environments Revealed by Enrichment Culture: Eun Hye Kim ^1,2, Kyeung Hee Cho ¹, Yung Mi Lee ¹, Joung Han Yim ¹, Hong Kum Lee ¹, Jang-Cheon Cho ², Soon Gyu Hong ¹; Journal of Microbiology 2010;48(4):426-432.
DOI: https://doi.org/10.1007/s12275-010-0015-z
Published online: August 20, 2010

¹Polar BioCenter, Korea Polar Research Institute, KORDI, Songdo Techno Park, Incheon 406-840, Republic of Korea, ²Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea¹Polar BioCenter, Korea Polar Research Institute, KORDI, Songdo Techno Park, Incheon 406-840, Republic of Korea, ²Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea

Corresponding author: Soon Gyu Hong , Tel: +82-32-260-6310,

Received: 11 January 2010 • Accepted: 3 May 2010

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Abstract

A new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from Svalbard, Norway. The method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. ZoBell and 0.1× nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. One to three types of colonies were observed from each enrichment culture, indicating that specific bacterial species were enriched during the experimental conditions. Among 89 bacterial isolates, protease activity was observed from 48 isolates in the screening media containing skim milk. Good growth was observed at 4°C and 10°C while none of the isolates could grow at 37°C. At low temperatures, enzyme activity was equal to or higher than activity at higher temperatures. Bacterial isolates were included in the genera Pseudoalteromonas (33 isolates), Arthrobacter (24 isolates), Pseudomonas (16 isolates), Psychrobacter (6 isolates), Sphingobacterium (6 isolates), Flavobacterium (2 isolates), Sporosarcina (1 isolate), and Stenotrophomonas (1 isolate). Protease activity was observed from Pseudoalteromonas (33 isolates), Pseudomonas (10 isolates), Arthrobacter (4 isolates), and Flavobacterium (1 isolate).

Keywords: Arctic;enrichment;protease

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Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial and Marine Environments Revealed by Enrichment Culture

J. Microbiol. 2010;48(4):426-432. Published online August 20, 2010

DOI: https://doi.org/10.1007/s12275-010-0015-z

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