Development of a Latex Agglutination Test for Norovirus Detection

Heetae Lee; YoungBin Park; Misoon Kim; Youngmee Jee; Doo-sung Cheon; Hae Sook Jeong; GwangPyo Ko

doi:10.1007/s12275-010-0071-4

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Research Support, Non-U.S. Gov't
Development of a Latex Agglutination Test for Norovirus Detection: Heetae Lee ¹, YoungBin Park ¹, Misoon Kim ¹, Youngmee Jee ², Doo-sung Cheon ², Hae Sook Jeong ², GwangPyo Ko ^1,3; Journal of Microbiology 2010;48(4):419-425.
DOI: https://doi.org/10.1007/s12275-010-0071-4
Published online: August 20, 2010

¹Department of Environmental Health, Institute for Health and Environment, School of Public Health, Seoul National University, Seoul 151-742, Republic of Korea, ²Division of Enteric and Hepatitis Viruses, Center for Infectious Disease, Korea National Institute of Health, Korea Center for Disease Control and Prevention, Seoul 122-701, Republic of Korea, ³Institute for Microbiology, School of Life Science, Seoul National University, Seoul 110-799, Republic of Korea

Corresponding author: GwangPyo Ko , Tel: +82-2-880-2731,

Received: 23 February 2010 • Accepted: 5 April 2010

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Abstract

Norovirus (NoV) is the leading cause of acute gastroenteritis worldwide. Currently, reverse transcription polymerase chain reaction (RT-PCR) is used commonly to detect NoVs in both clinical and environmental samples. However, RT-PCR requires expensive equipment and cannot be performed on site. In this study, a latex agglutination test (LAT) using antibody-labeled latex beads for detecting NoVs was developed. Two kinds of polyclonal antibodies, one generated from synthetic peptides and the other from E. coli-expressed NoV capsid proteins, were used to develop the LAT. Each of these polyclonal antibodies was immobilized on the surface of latex beads and tested for the ability to detect NoVs. Under optimized conditions, our LAT detected GII.4 NoV at concentrations as low as 3.3×105 RT-PCR units/ml in stool samples. The detection limit for the LAT was approximately 1.7×103 RT-PCR units. Forty-eight stool samples were tested for NoVs using this LAT. In comparison with an RT-PCR assay, the sensitivity and specificity of the LAT were 35% and 100%, respectively. With further optimization, this LAT used with appropriate antibodies could be applied for convenient detection of NoVs in clinical diagnosis and food monitoring.

Keywords: polyclonal antibody;latex agglutination test (LAT);norovirus;rapid detection;sensitivity;specificity

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Development of a Latex Agglutination Test for Norovirus Detection

J. Microbiol. 2010;48(4):419-425. Published online August 20, 2010

DOI: https://doi.org/10.1007/s12275-010-0071-4

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