Molecular Characteristics and Resistant Mechanisms of Imipenem-Resistant Acinetobacter baumannii Isolates in Shenyang, China

Jing Ping Zhang; Wan Zhu; Su Fei Tian; Yun Zhuo Chu; Bai Yi Chen

doi:10.1007/s12275-010-0137-3

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Molecular Characteristics and Resistant Mechanisms of Imipenem-Resistant Acinetobacter baumannii Isolates in Shenyang, China: Jing Ping Zhang ¹, Wan Zhu ², Su Fei Tian ³, Yun Zhuo Chu ³, Bai Yi Chen ¹; Journal of Microbiology 2010;48(5):689-694.
DOI: https://doi.org/10.1007/s12275-010-0137-3
Published online: November 3, 2010

¹Division of Infectious Diseases, ²Department of Nosocomial Infection Control, ³Department of Laboratory Medicine, The First Hospital of China Medical University, Shenyang, 110001, P. R. China

Corresponding author: Jing Ping Zhang , Tel: +86-24-8328-2552,

Received: 12 April 2010 • Accepted: 17 June 2010

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Abstract

The investigation was carried out to elucidate the molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii. Thirty-seven isolates were collected from January 2007 to December 2007. The homology of the isolates was analyzed by both pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The genes of β-lactamases, adeB, and class 1 integron were polymerase chain reaction amplified. Genotype analysis of the 37 A. baumannii isolates by PFGE revealed the circulation of four PFGE types (A-D) ; the A- and B-type accounted for 48.6% and 40.5%, respectively. MLST showed the existence of three allelic profiles. The agar dilution method was carried out to determine the MIC of imipenem, in the absence or presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP, 10 μg/ml). The MICs of the strains to imipenem were between 16 μg/ml and 128 μg/ml. When CCCP was added, a MIC decrease of at least four-fold was observed in 20 isolates, which belonged to the A- or C-type. AdeB and blaPER-1 genes were each detected in 35 isolates, blaOXA-23 gene in 34 isolates and blaOXA-58-like gene in 24 isolates. All isolates harbored blaOXA-51-like genes. No isolates carried the blaIMP-1 gene. Integron was detected in 25 isolates, which mediated the resistance to aminoglycosides and rifampin. The epidemiologic data suggested that the increasing infection of A. baumannii in our hospital was mainly caused by the inter-hospital spread of two epidemic clones. The AdeABC efflux system may be the important factor that leads to the high level of imipenem-resistance in PFGE A-type.

Keywords: A. baumannii;PFGE;MLST;β-lactamase;efflux system;integron

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Molecular Characteristics and Resistant Mechanisms of Imipenem-Resistant Acinetobacter baumannii Isolates in Shenyang, China

J. Microbiol. 2010;48(5):689-694. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-0137-3

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