Molecular Cloning and Characterization of clyA Genes in Various Serotypes of Salmonella enterica

Lan Ji Huang; Jinghua Cui; Hong Hua Piao; Yeongjin Hong; Hyon E. Choy; Phil Youl Ryu

doi:10.1007/s12275-010-9268-9

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Molecular Cloning and Characterization of clyA Genes in Various Serotypes of Salmonella enterica: Lan Ji Huang ^1,2, Jinghua Cui ^1,2, Hong Hua Piao ^1,3, Yeongjin Hong ^1,2,3, Hyon E. Choy ^1,2,3, Phil Youl Ryu ^1,2,4; Journal of Microbiology 2010;48(5):663-667.
DOI: https://doi.org/10.1007/s12275-010-9268-9
Published online: November 3, 2010

¹Department of Microbiology, ²Brain Korea 21 Project, Center for Biomedical Human Resources, ³Research Center for Controlling Microbial Virulence, Chonnam National University Medical School, ⁴Chonnam National University Research Institute of Medical Sciencies, Gwangju 501-746, Republic of Korea

Corresponding author: Phil Youl Ryu , Tel: +82-62-220-4135,

Received: 27 August 2009 • Accepted: 8 June 2010

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Abstract

Cytolysin A (ClyA) is a pore-forming hemolytic protein encoded by the clyA gene. It has been identified in Salmonella enterica serovars Typhi and Paratyphi A. To identify and characterize the clyA genes in various Salmonella enterica strains, 21 different serotypes of strains isolated from clinical specimens were presently examined. Full-length clyA genes were found in S. enterica serovar Brandenburg, Indiana, Panama, and Schwarzengrund strains by polymerase chain reaction amplification. The ClyA proteins from these four strains showed >97% amino acid identity to that of S. enterica serovar Typhi. Although all four serovars expressed detectable levels of ClyA as determined by Western blot analysis, they did not show a strong hemolytic effect on blood agar, indicating that ClyA may not be efficiently expressed or secreted. Escherichia coli transformed with clyA genes from the four serovars enhanced production of ClyA proteins and hemolytic activities to a level similar to S. enterica serovar Typhi ClyA. The present results suggest that ClyA may play a role in the pathogenesis of S. enterica serovar Brandenburg, Indiana, Panama and Schwarzengrund.

Keywords: ClyA;cytolysin;pore-forming toxin;Salmonella

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Molecular Cloning and Characterization of clyA Genes in Various Serotypes of Salmonella enterica

J. Microbiol. 2010;48(5):663-667. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-9268-9

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