Replication and Pathogenesis of the Pandemic (H1N1) 2009 Influenza Virus in Mammalian Models

Donghyok Kwon; Kyeongcheol Shin; Seungtae Kim; Yooncheol Ha; Jang-Hoon Choi; Jeong Seon Yang; Joo-Yeon Lee; Chanhee Chae; Hee-Bok Oh; Chun Kang

doi:10.1007/s12275-010-0120-z

Articles

Page Path: HOME > J. Microbiol > Volume 48(5); 2010 > Article

Research Support, Non-U.S. Gov't
Replication and Pathogenesis of the Pandemic (H1N1) 2009 Influenza Virus in Mammalian Models: Donghyok Kwon ^1,2, Kyeongcheol Shin ¹, Seungtae Kim ³, Yooncheol Ha ^1,2, Jang-Hoon Choi ¹, Jeong Seon Yang ³, Joo-Yeon Lee ¹, Chanhee Chae ², Hee-Bok Oh ¹, Chun Kang ¹; Journal of Microbiology 2010;48(5):657-662.
DOI: https://doi.org/10.1007/s12275-010-0120-z
Published online: November 3, 2010

¹Division of Influenza Viruses, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea, ²Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea, ³Division of Respiratory Viruses, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea

Corresponding author: Chun Kang , Tel: +82-2-380-2100;,

Received: 1 April 2010 • Accepted: 17 June 2010

prev next

282 Views
0 Download
18 Crossref
20 Scopus

Full Article

Download PDF

Abstract

This study aimed to characterize the replication and pathogenic properties of a Korean pandemic (H1N1) 2009 influenza virus isolate in ferrets and mice. Ferrets infected with A/Korea/01/2009 (H1N1) virus showed mild clinical signs. The virus replicated well in lungs and slightly in brains with no replication in any other organs. Severe bronchopneumonia and thickening of alveolar walls were detected in the lungs. Viral antigens were detected in the bronchiolar epithelial cells, in peribronchial glands with severe peribronchitis and in cells present in the alveoli. A/Korea/01/2009 (H1N1) virus-infected mice showed weight loss and pathological lung lesions including perivascular cuffing, interstitial pneumonia and alveolitis. The virus replicated highly in the lungs and slightly in the nasal tissues. Viral antigens were detected in bronchiolar epithelial cells, pneumocytes and interstitial macrophages. However, seasonal H1N1 influenza virus did not replicate in the lungs of ferrets, and viral antigens were not detected. Thus, this Korean pandemic (H1N1) 2009 isolate infected the lungs of ferrets and mice successfully and caused more pathological lesions than did the seasonal influenza virus.

Keywords: pandemic (H1N1) 2009 virus;influenza;ferrets;mice;pathogenesis;immunohistochemistry

Figure & Data

References

Citations

Citations to this article as recorded by

Comparative pathogenicity of influenza virus-induced pneumonia mouse model following intranasal and aerosolized intratracheal inoculation
Xiu-Yu Jin, Hui-Ying Yang, Guang-Yu Zhao, Chen-Xi Dai, Zai-Qing Zhang, Dong-Sheng Zhou, Qi Yin, Er-Hei Dai
Virology Journal.2024;[Epub] CrossRef
Pathogenesis and transmission of human seasonal and swine-origin A(H1) influenza viruses in the ferret model
Joanna A. Pulit-Penaloza, Nicole Brock, Joyce Jones, Jessica A. Belser, Yunho Jang, Xiangjie Sun, Sharmi Thor, Claudia Pappas, Natosha Zanders, Terrence M. Tumpey, C. Todd Davis, Taronna R. Maines
Emerging Microbes & Infections.2022; 11(1): 1452. CrossRef
Histological Evidence of Pulmonary Microthrombosis and Vasculitis in Life-Threatening Respiratory Virus Diseases
Heather W Dolby, Philippe Potey, Annika B Wilder-Smith, Sara Clohisey, Jonathan E Millar, J Kenneth Baillie, David A Dorward, Christopher D Lucas, Clark D Russell
Open Forum Infectious Diseases.2021;[Epub] CrossRef
In Vivo Models to Study the Pathogenesis of Extra-Respiratory Complications of Influenza A Virus Infection
Edwin Veldhuis Kroeze, Lisa Bauer, Valentina Caliendo, Debby van Riel
Viruses.2021; 13(5): 848. CrossRef
Evaluation of Protective Immunity of Peptide Vaccines Composed of a 15-mer N-terminal Matrix Protein 2 and a Helper T-Cell Epitope Derived from Influenza A Virus
Jeong-Ki Kim, Sinyoung Cheong, Myung Kyu Lee
Immune Network.2019;[Epub] CrossRef
Sowing the Seeds of a Pandemic? Mammalian Pathogenicity and Transmissibility of H1 Variant Influenza Viruses from the Swine Reservoir
Joanna A. Pulit-Penaloza, Jessica A. Belser, Terrence M. Tumpey, Taronna R. Maines
Tropical Medicine and Infectious Disease.2019; 4(1): 41. CrossRef
Inhibition of Influenza Virus Replication by DNA Aptamers Targeting a Cellular Component of Translation Initiation
Paloma Rodriguez, M Isabel Pérez-Morgado, Víctor M Gonzalez, M Elena Martín, Amelia Nieto
Molecular Therapy - Nucleic Acids.2016; 5: e308. CrossRef
Prevalence of gastrointestinal symptoms in patients with influenza, clinical significance, and pathophysiology of human influenza viruses in faecal samples: what do we know?
Laetitia Minodier, Remi N. Charrel, Pierre-Emmanuel Ceccaldi, Sylvie van der Werf, Thierry Blanchon, Thomas Hanslik, Alessandra Falchi
Virology Journal.2015;[Epub] CrossRef
Genome Wide Identification of SARS-CoV Susceptibility Loci Using the Collaborative Cross
Lisa E. Gralinski, Martin T. Ferris, David L. Aylor, Alan C. Whitmore, Richard Green, Matthew B. Frieman, Damon Deming, Vineet D. Menachery, Darla R. Miller, Ryan J. Buus, Timothy A. Bell, Gary A. Churchill, David W. Threadgill, Michael G. Katze, Leonard
PLOS Genetics.2015; 11(10): e1005504. CrossRef
Mammalian pathogenesis of oseltamivir-resistant pandemic (H1N1) 2009 influenza virus isolated in South Korea
Donghyok Kwon, Kyeongcheol Shin, Su-Jin Kim, Joo-Yeon Lee, Chun Kang
Virus Research.2014; 185: 41. CrossRef
Animal models for influenza virus pathogenesis, transmission, and immunology
Rajagowthamee R. Thangavel, Nicole M. Bouvier
Journal of Immunological Methods.2014; 410: 60. CrossRef
Experimental infection and natural contact exposure of ferrets with canine influenza virus (H3N2)
Yu-Na Lee, Dong-Hun Lee, Jae-Keun Park, Seong-Su Yuk, Jung-Hoon Kwon, Sang-Soep Nahm, Joong-Bok Lee, Seung-Yong Park, In-Soo Choi, Chang-Seon Song
Journal of General Virology .2013; 94(2): 293. CrossRef
DBA/2 mouse as an animal model for anti-influenza drug efficacy evaluation
Jin Il Kim, Sehee Park, Sangmoo Lee, Ilseob Lee, Jun Heo, Min-Woong Hwang, Joon-Yong Bae, Donghwan Kim, Seok-Il Jang, Mee Sook Park, Man-Seong Park
Journal of Microbiology.2013; 51(6): 866. CrossRef
Characterization In Vitro and In Vivo of a Pandemic H1N1 Influenza Virus from a Fatal Case
Ariel Rodriguez, Ana Falcon, Maria Teresa Cuevas, Francisco Pozo, Susana Guerra, Blanca García-Barreno, Pamela Martinez-Orellana, Pilar Pérez-Breña, Maria Montoya, Jose Antonio Melero, Manuel Pizarro, Juan Ortin, Inmaculada Casas, Amelia Nieto, Andreas Zi
PLoS ONE.2013; 8(1): e53515. CrossRef
Comparison of Children Hospitalized With Seasonal Versus Pandemic Influenza A, 2004–2009
Dat Tran, Wendy Vaudry, Dorothy L. Moore, Julie A. Bettinger, Scott A. Halperin, David W. Scheifele, Samina Aziz
Pediatrics.2012; 130(3): 397. CrossRef
Susceptibility of human H3N2 influenza virus to oseltamivir in South Korea, 2009–2011
Sehee Park, Jin Il Kim, Ilseob Lee, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Eun-Joo Lim, Won-Seok Seok, Hee Jin Cheong, Joon Young Song, Woo Joo Kim, Man-Seong Park
Journal of Microbiology.2012; 50(6): 1067. CrossRef
Influenza A(H1N1)pdm09 Virus Suppresses RIG-I Initiated Innate Antiviral Responses in the Human Lung
Wenxin Wu, Wei Zhang, J. Leland Booth, Jordan P. Metcalf, Michael CW. Chan
PLoS ONE.2012; 7(11): e49856. CrossRef
Pandemic H1N1 influenza virus causes a stronger inflammatory response than seasonal H1N1 influenza virus in ferrets
Young Myong Kang, Byung Min Song, Joo Sub Lee, Hyun Soo Kim, Sang Heui Seo
Archives of Virology.2011; 156(5): 759. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Replication and Pathogenesis of the Pandemic (H1N1) 2009 Influenza Virus in Mammalian Models

J. Microbiol. 2010;48(5):657-662. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-0120-z

Related articles