Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.)

Young Tae Kim; Myoungho Cho; Je Yong Jeong; Hyang Burm Lee; Seung Bum Kim

doi:10.1007/s12275-010-0126-6

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Research Support, Non-U.S. Gov't
Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.): Young Tae Kim ¹, Myoungho Cho ¹, Je Yong Jeong ², Hyang Burm Lee ², Seung Bum Kim ¹; Journal of Microbiology 2010;48(5):566-572.
DOI: https://doi.org/10.1007/s12275-010-0126-6
Published online: November 3, 2010

¹Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea, ²Environmental Microbiology Lab, Division of Applied Bioscience and Biotechnology, Chonnam National University, Gwangju 500-757, Republic of Korea

Corresponding author: Seung Bum Kim , Tel: +82-42-821-6412,

Received: 2 April 2010 • Accepted: 14 June 2010

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Abstract

Microbial communities in hot pepper (Capsicum annuum L.) cultivation fields under different cultivation methods were investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis. Rhizosphere soil and leaf samples were collected from control, conventional and nature-friendly cultivation fields between May and July, 2009. Two Bacillus subtilis strains were applied to nature-friendly cultivation fields as biocontrol agents during the sampling period. Relative abundances of bacteria and plant pathogenic fungi related T-RFs were also measured to monitor the effect of biocontrol agents on potential plant pathogenic fungi. In the principal component analysis (PCA) based on T-RFLP profiles, the microbial communities from rhizosphere soil samples in July, including bacteria and fungi, showed distinct difference between nature-friendly cultivation fields and other cultivation fields. However, there was no correlation between cultivation methods and leaf microbial communities at any sampling period. Changes in the abundance of bacteria related T-RF in the rhizosphere of nature-friendly cultivation fields were observed clearly two months after application of biocontrol agent, while the abundance of plant pathogenic fungi related T-RFs significantly decreased.

Keywords: terminal restriction fragment length polymorphism;nature-friendly cultivation;B. subtilis;biocontrol

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Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.)

J. Microbiol. 2010;48(5):566-572. Published online November 3, 2010

DOI: https://doi.org/10.1007/s12275-010-0126-6

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